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Acesso livre e os desafios do conhecimento : o caso da Universidade do Minho

Apresentação efectuada no "Congresso Internacional SIBi Usp 30 anos : o futuro do conhecimento universal", S. Paulo, Brasil, 07-08 Outubro 2011.

Changes on surface morphology of corn starch blend films

This study aims at evaluating the influence of enzymatic degradation solution on the surface morphology and thermal properties of a poly(ethylene-vinyl alcohol) copolymer-corn starch thermoplastic blend (SEVA-C), as a function of immersion time. To perform this study, three different batches were assessed using SEVA-C samples of different thicknesses and a fixed weight of 1.6 g, immersed in alpha-amylase (50 u/...

Exploiting the sequence of naturally occurring elastin : construction, producti...

Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A synthetic monomer DNA sequence encoding for (VPAVG)20, was used to build a library of concatemer genes with precise control on sequence and size. The higher molecular weight polymer with 220 repeats of VPAVG was biologi...

Proteolytic enzyme engineering : a tool for wool

One of the goals of protein engineering is to tailor the structure of enzymes to optimize industrial bioprocesses. In the present work, we present the construction of a novel high molecular weight subtilisin, based on the fusion of the DNA sequences coding for Bacillus subtilis prosubtilisin E and for an elastin-like polymer (ELP). The resulting fusion protein was biologically produced in Escherichia coli, puri...

Recombinant elastin‐like polymers : from the design towards application

Enzymatic degradation of starch thermoplastic blends using samples of different...

The material studied was a thermoplastic blend of corn starch with a poly(ethylene-vinyl alcohol) copolymer, SEVA-C. The influence of both the material’s exposed surface and enzyme concentration on degradation kinetics was studied. As α-amylase is present in the blood plasma, experiments were performed, varying the material thickness and the α-amylase between 50 and 100 units/l, at 37°C, lasting up to 90 days. ...

Construction and bioproduction of a "green" synthetic protein-based polymer exh...

Changes on surface morphology of starch based materials after enzymatic degrada...

Changes on surface morphology of starch based thermoplastic blends after enzyma...

Perfil de isolamento microbiano em cães com otite média e externa associadas

Comparou-se o perfil de isolamento microbiano de amostras coletadas de cães com otite média (OM) e externa (OE) associadas. Sessenta e quatro cães com otite média e externa foram avaliados durante 10 meses. Amostras dos condutos auditivos externos foram coletadas com auxílio de swabs estéreis e aquelas do ouvido médio pela técnica cirúrgica da osteotomia da bula timpânica. Os microrganismos foram cultivados e i...