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Somatic embryogenesis and plant regeneration in carob ( Ceratonia siliqua L.)
Canhoto, Jorge; Rama, Sandra; Cruz, GilSummary Somatic embryos of carob (Ceratonia siliqua L.) were induced from cotyledonary segments excised from immature seeds when cultured on Murashige and Skoog media supplemented with several combinations of 6-benzylaminopurine (BA) and indole-3-butyric acid (IBA). The best frequencies of induction (33.8%) were obtained when 4.4 µM BA and 0.5 µM IBA were used. Shoots were also sporadically formed in the same ...
Somatic embryogenesis and plant regeneration in myrtle (Myrtaceae)
Canhoto, Jorge; Lopes, Maria; Cruz, GilAbstract Somatic embryos of myrtle (Myrtus communis L.) were induced from mature zygotic embryos cultured in MS medium supplemented with several concentrations of 2,4-D (2.26 µM – 18.98 µM) or Picloram (2.07 µM – 16.5 µM) combined with 0.087 M or 0.23 M sucrose. For all the concentrations of 2,4-D or Picloram tested, 0.087 M sucrose proved to be more effective than 0.23 M. The best frequencies of induction wer...
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