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Multigene Molecular Systematics Confirm Species Status of Morphologically Conve...
Silva, Joana Matzen da; Santos, Antonina dos; Cunha, Marina R.; Costa, Filipe O.; Creer, Simon; Carvalho, Gary R.Introduction: In spite of contemporary morphological taxonomy appraisals, apparent high morphological similarity raises uncertainty about the species status of certain Pagurus hermit crabs. This is exemplified between two European species, Pagurus excavatus (Herbst, 1791) and Pagurus alatus (Fabricius 1775), whose species status is still difficult to resolve using morphological criteria alone. Methodology/Princ...
Isolation of marine meiofauna from sandy sediments From decanting to DNA extrac...
Fonseca, Vera; Packer, Margaret; Carvalho, Gary; Power, Deborah; Lambshead, John; Creer, SimonThis protocol describes the separation of marine meiofauna from sediment and subsequent environmental DNA extraction. In this study meiofauna samples were taken with a 45 mm core from the upper 5 to 10 cm of sediment layer. Separation from sediment was achieved using a decantation process followed by isolation from fine silt using repetitive centrifugation steps with a 1.16 specific gravity (sg) LUDOX-TM soluti...
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