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Analytical methods applied to the chemical characterization and antioxidant pro...

Reis, Filipa S.; Barros, Lillian; Sousa, Maria João; Martins, Anabela; Ferreira, Isabel C.F.R.

The chemical composition and the antioxidant potential of three species of wild mushrooms from Northeastern Portugal, namely Agaricus albertii, Agaricus urinascens var. excellens, and Pleurotus eryngii, were compared. Standard procedures were followed in the nutritional value evaluation, while chromatographic procedures were used to analyze free sugars, fatty acids, tocopherols, phenolic compounds, and organic ...

Data: 2014   |   Origem: Biblioteca Digital do IPB

Uso da aromaterapia no controlo de stresse e ansiedade

Dias, Paula; Sousa, Maria João; Pereira, Olívia R.

As terapias alternativas e/ou complementares surgem como terapias importantes na prevenção e tratamento de diversos problemas de saúde, de entre o quais o stresse e ansiedade, problemas cada vez mais frequentes na sociedade atual. O presente estudo acerca da utilização de produtos naturais em aromaterapia pretendeu avaliar a eficácia da aromaterapia na diminuição dos níveis de stresse e ansiedade, através da té...

Data: 2014   |   Origem: Biblioteca Digital do IPB

Preliminary critical nitrogen nutritional indices for lemon balm (Melissa offic...

Freitas, Sara; Arrobas, Margarida; Ferreira, I.Q.; Sousa, Maria João; Rodrigues, M.A.

The management of crop fertilization presupposes previous diagnoses of soil fertility and plant nutritional status. In Europe, regular soil testing and plant analysis is mandatory for farmers receiving EU subsidies. Soil testing provides information on the potential availability of nutrients in the soil, a key component in the fertilization recommendation systems. By analyzing plant tissues we can access the nu...

Data: 2014   |   Origem: Biblioteca Digital do IPB

Genome-wide identification of genes involved in the positive and negative regul...

Sousa, Marlene; Duarte, Ana Marta Gomes; Fernandes, Tânia Alícia Ribeiro; Chaves, S. R.; Pacheco, Andreia; Leão, Cecília; Côrte-Real, Manuela

Background: Acetic acid is mostly known as a toxic by-product of alcoholic fermentation carried out by Saccharomyces cerevisiae, which it frequently impairs. The more recent finding that acetic acid triggers apoptotic programmed cell death (PCD) in yeast sparked an interest to develop strategies to modulate this process, to improve several biotechnological applications, but also for biomedical research. Indeed,...


C2-phytoceramide perturbs lipid rafts and cell integrity in Saccharomyces cerev...

Pacheco, Andreia; Azevedo, Flávio; Rego, António; Santos, Júlia; Chaves, S. R.; Côrte-Real, Manuela; Sousa, Maria João

Specific ceramides are key regulators of cell fate, and extensive studies aimed to develop therapies based on ceramide-induced cell death. However, the mechanisms regulating ceramide cytotoxicity are not yet fully elucidated. Since ceramides also regulate growth and stress responses in yeast, we studied how different exogenous ceramides affect yeast cells. C2-phytoceramide, a soluble form of phytoceramides, the...


Ammonium-dependent shortening of CLS in yeast cells starved for essential amino...

Santos, Júlia; Leão, Cecília; Sousa, Maria João

Ammonium (NH4(+)) leads to chronological life span (CLS) shortening in Saccharomyces cerevisiae BY4742 cells, particularly evident in cells starved for auxotrophy-complementing amino acids (leucine, lysine, and histidine) simultaneously. Here, we report that the effect of NH4(+) on aging yeast depends on the specific amino acid they are deprived of. Compared with no amino acid starvation, starvation for leucine...


The genome sequence of the highly acetic acid-tolerant zygosaccharomyces bailii...

Leão, Cecília; Rodrigues, Fernando José dos Santos; Santos, Júlia; Mira, Nuno P.; Münsterkötter, Martin; Valada, Filipa Dias; Palma, Margarida

In this work, it is described the sequencing and annotation of the genome of the yeast strain ISA1307, isolated from a sparkling wine continuous production plant. This strain, formerly considered of the Zygosaccharomyces bailii species, has been used to study Z. bailii physiology, in particular, its extreme tolerance to acetic acid stress at low pH. The analysis of the genome sequence described in this work ind...


Phenolic profiles of cultivated, in vitro cultured and commercial samples of Me...

Barros, Lillian; Dueñas, Montserrat; Dias, Maria Inês; Sousa, Maria João; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.

Melissa officinalis L. (lemon balm) is normally consumed as infusions and presents therapeutic properties such as sedative, carminative and antispasmodic, being also included in some pharmaceutical preparations. The phenolic profiles of different samples of lemon balm prepared as infusions were evaluated by HPLC-DAD-ESI/MS. The profiles were compared in order to understand the differences between cultivated, in...

Data: 2013   |   Origem: Biblioteca Digital do IPB

Portuguese wild mushrooms at the "Pharma-Nutrition" interface: nutritional char...

Leal, Ana Raquel; Barros, Lillian; Barreira, João C.M.; Sousa, Maria João; Martins, Anabela; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.

The search for foods that might improve health or reduce disease risk, has been progressively gaining interest. Mushroom could be examples of these foods, presenting the additional advantage of being recognized as a delicacy. This feature might place mushrooms in the pharma-nutrition interface. Herein, eight different mushroom species were characterized in terms of nutrients (proteins, carbohydrates, fat, indiv...

Data: 2013   |   Origem: Biblioteca Digital do IPB

Chemical characterization and antioxidant properties of Lepista nuda fruiting b...

Pinto, Sara; Barros, Lillian; Sousa, Maria João; Ferreira, Isabel C.F.R.

Lepista nuda is an edible mushroom which presents important organoleptic qualities including a delicate flavor and good postharvest conservation. Its chemical and bioactive properties can be affected by habitat collection. Therefore, the main goal of the present work was to compare chemical composition and antioxidant potential of Lepista nuda samples from different habitats, and mycelia produced by in vitro cu...

Data: 2013   |   Origem: Biblioteca Digital do IPB

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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia