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Flow cytometry as a tool to assess the effects of gamma radiation on the viabil...

Mesquita, N.; Portugal, A.; Piñar, G.; Loureiro, J.; Coutinho, A. P.; Trovão, J.; Nunes, I.; Botelho, M. L.; Freitas, H.

Flow cytometry is often used for viability and vitality assessment in bacteria and yeasts. However, its application to the study of fungal spore development is uncommon, probably due to the difficulties in successfully staining these cells. In the current study, we used flow cytometry for the first time to assess the effects of a disinfection treatment on the survival, growth and metabolic activity of fungal sp...


IFNAR1 Controls Progression to Cerebral Malaria in Children and CD8+ T Cell Bra...

Ball, E. A.; Sambo, M. R.; Martins, M.; Trovoada, M. J.; Benchimol, C.; Costa, J.; Antunes Goncalves, L.; Coutinho, A.; Penha-Goncalves, C.

Malaria in pregnancy is exquisitely aggressive, causing a range of adverse maternal and fetal outcomes prominently linked to Plasmodium-infected erythrocyte cytoadherence to fetal trophoblast. To elucidate the physiopathology of infected erythrocytes (IE) sequestration in the placenta we devised an experimental system for intravital placental examination of P. berghei-infected mice. BALB/c females were mated to...


Sustained relief of obstructive symptoms for the remaining life of patients fol...

Canena, J; Liberato, M; Marques, I; Rodrigues, C; Lagos, A; Patrocínio, S; Tomé, M; Pires, E; Romão, C; Coutinho, A; Veiga, P; Neves, B; Deus, JR

BACKGROUND: self-expanding metal stents are currently being used as a definitive palliative treatment for malignant colorectal obstruction in patients with incurable disease. Few studies have followed large numbers of patients from stent placement until death, and those few have reported conflicting results in the long-term clinical outcome data. AIMS: this study evaluated the clinical effectiveness of stent p...


Occupational therapy in spinocerebellar ataxia type 3: an open-label trial

Silva,R.C.R.; Saute,J.A.M.; Silva,A.C.F.; Coutinho,A.C.O.; Saraiva-Pereira,M.L.; Jardim,L.B.

Occupational therapy (OT) is a profession concerned with promoting health and well-being through occupation, by enabling handicapped people to participate in the activities of everyday life. OT is part of the clinical rehabilitation of progressive genetic neurodegenerative diseases such as spinocerebellar ataxias; however, its effects have never been determined in these diseases. Our aim was to investigate the ...

Data: 2010   |   Origem: OASIS br

Manejo da irrigação em pivôs centrais no cerrado de Minas Gerais.

ALBUQUERQUE, P. E. P. de; COUTINHO, A. C.; ANDRADE, C. de L. T. de; GUIMARAES, D. P.; DUARTE, J. de O.

2010

Data: 2010   |   Origem: OASIS br

Increased BDNF levels and NTRK2 gene association suggest a disruption of BDNF/T...

Correia, C.T.; Coutinho, A.M.; Sequeira, A.F.; Sousa, I.G.; Lourenço Venda, L.; Almeida, J.P.; Abreu, R.L.; Lobo, C.; Miguel, T.S.; Conroy, J.

The brain-derived neurotrophic factor (BDNF), a neurotrophin fundamental for brain development and function, has previously been implicated in autism. In this study, the levels of BDNF in platelet-rich plasma were compared between autistic and control children, and the role of two genetic factors that might regulate this neurotrophin and contribute to autism etiology, BDNF and NTRK2, was examined. We found that...


Utilização de fixação externa em fratura de úmero em caprino

Vechiato,T.A.F.; Siqueira,R.F.; Coutinho,A.; Marchioni,G.G.; Kolber,M.; Peixoto Jr.,K.C.; Toffoli,P.Z.; Zanco,N.A.

Data: 2009   |   Origem: OASIS br

Avaliação do ruído de tráfego : metodologia para a caracterização de camadas de...

Antunes, M. L.; Coutinho, A. S.; Patrício, J.; Freitas, Elisabete F.; Paulo, Joel; Coelho, J. L. Bento; Cardoso, F. T.

Uma das consequências do desenvolvimento económico verificado nas últimas décadas é o aumento significativo do tráfego rodoviário. O ruído induzido pelo tráfego é um importante factor de poluição ambiental que afecta cada vez um maior número de pessoas. Nesta comunicação efectua-se a comparação do nível de ruído (Lmax) registado para os veículos ligeiros, e pesados, de acordo com o método estatístico de passage...


A contribution to the ultrastructural knowledge of the pollen exine in subtribe...

Pereira Coutinho, A.; Dinis, A. M.

Abstract To better understand the relationships within the Asteroideae and Inuleae, the structure of the pollen exine was investigated in seven genera and nine species of the subtribe Inulinae using LM, TEM and SEM. All taxa have a senecioid pattern of exine. The tectal complex consists of three main layers that differ in thickness and morphology: a tectum, a columellar layer, and a layer consisting of the bas...


Is the quantity of orbicules released by Dactylis glomerataand Cynosurus echina...

Dinis, Augusto M.; Baptista, Filipa; Coutinho, A. Pereira

Orbicule characteristics of Dactylis glomerataand Cynosurus echinatus(Poaceae) were investigated using light (LM), scanning electron (SEM) and transmission electron microscopy (TEM). Based on SEM micrographs, the number of orbicules per 100 µm2 of the locule wall surface was determined in both dehisced and undehisced anthers and was further compared statistically. A total of 100 pollen grains were examined usin...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia