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Nanostructured thin coatings from chitosan and an elastin-like recombinamer wit...
Costa, Rui R.; Ribeiro, Artur J.; Rodríguez-Cabello, José Carlos; Mano, J. F.Publicado em: Materials Science Forum, vols. 730-732; Online available since 2012/Nov/12 ; In the present work, chitosan (CHI) and elastin-like recombinamers (ELRs) were used to conceive nanostructured thin films driven by sequential electrostatic layer-by-layer (LbL), a simple and versatile technique that discards the use of harmful reagents. Two similar ELRs were engineered to contain negatively charged amin...
Nanostructured multilayer compartments : towards multifunctionality and ‘‘cell-...
Costa, Rui R.; Castro, E.; Arias, F. J.; Rodríguez-Cabello, José Carlos; Mano, J. F.In living organisms, there are phenomena that require the presence of specific biomolecules with distinct function and in variable concentra- tions at a given time, such as the healing and regeneration of tissue and organ lesions. In this work, we propose the use of a compartment- ed drug delivery device for the multiple release of bioactive agents. It consists of nanostructured microcapsules confined within a ...
Self-assembled microcapsules with tunable and sustained release of macromolecul...
Costa, Rui R.; Custódio, Catarina A.; Arias, F. J.; Rodríguez-Cabello, José Carlos; Mano, J. F.Layer-by-layer (LbL) has been presented as a tool capable of constructing tunable and sustained release reservoirs for therapeutic and proliferation/differentiation agents. We report the conception of biocompatible stimuli-responsive microcapsules fabricated using LbL containing BSA as model protein. The capsules were constructed by the sequential adsorption of chitosan and a temperature-responsive elastin-like...
Nanostructured multilayer compartments : towards multifunctionality and ‘‘cell-...
Costa, Rui R.; Castro Otero, Emilio; Arias, F. J.; Rodríguez-Cabello, José Carlos; Mano, J. F.In living organisms, there are phenomena that require the presence of specific biomolecules with distinct function and in variable concentrations at a given time, such as the healing and regeneration of tissue and organ lesions. In this work, we propose the use of a compartmented drug delivery device for the multiple release of bioactive agents. It consists of nanostructured microcapsules confined within a mill...
Three-dimensional layer-by-layer strategies for tissue engineering and nanomedi...
Costa, Rui R.; Mano, J. F.Layer-by-layer (LbL) is a self-assembly-driven surface modification strategy that allows the construction of nanostructured films onto substrates of any geometry, from simple bidimensional surfaces to more complex three-dimensional porous scaffolds. The principle behind LbL lies in the existence of multiple intermolecular interactions, such as electrostatic contacts, hydrophobic interactions, and hydrogen bondi...
Nanostructured multilayers in the production of new devices for biomedical appl...
Costa, Rui R.; Mano, J. F.Surface engineering is of the utmost significance in the conception of devices with an improved biological performance. By addressing physical and chemical features of interfaces, it has been possible to develop patterned and stimuli-responsive devices with tunable wettability and protein/cell adhesion properties with application in biomedicine and tissue engineering. While several surface engineering approache...
Layer-by-layer assembly of chitosan and recombinant biopolymers into biomimetic...
Costa, Rui R.; Custódio, Catarina A.; Arias, F. J.; Rodríguez-Cabello, José Carlos; Mano, J. F.In this work, biomimetic smart thin coatings using chitosan and a recombinant elastin-like recombinamer (ELR) containing the cell attachment sequence arginine–glycine–(aspartic acid) (RGD) are fabricated through a layer-by-layer approach. The synthetic polymer is characterized for its molecular mass and composition using mass spectroscopy and peptide sequencing. The adsorption of each polymeric layer is followe...
Temperature-responsive microcapsules prepared by nano-structured multilayers of...
Costa, Rui R.; Arias, F. J.; Rodríguez-Cabello, José Carlos; Mano, J. F.Objectives: Polyelectrolyte vesicles using layer-by-layer (LbL) were recently intro-duced for the encapsulation of therapeutic molecules. This work presents multilay-ered microcapsules of chitosan and a temperature-responsive elastin-like recom-binamer (ELR) as a novel drug delivery system. The release of a pre-loaded model protein was studied at distinct temperatures and number of layers to evaluate the perm...
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