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Optimized extraction of a lectin from Crataeva tapia bark using AOT in isooctan...
Nascimento, Cynthia O.; Costa, Romero M. P. B.; Araújo, Regina M. S.; Chaves, Maria E. C.; Coelho, L. C. B. B.; Paiva, Patrícia M. G.; Teixeira, J. A.Crataeva tapia bark lectin was extracted from a crude extract into a reversed micelle phase of the anionic surfactant AOT in isooctane and back-extracted, to a final aqueous phase by addition of butanol. The effects of pH, ionic strength and surfactant concentration on the protein transfer process from the aqueous to the organic phase were characterized, being the best results obtained after 5 min of contact, u...
Data: 2008
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Origem: RepositóriUM - Universidade do Minho
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