Encontrados 3 documentos, a visualizar página 1 de 1
The alternative oxidase family of Vitis vinifera reveals an attractive model to...
Costa, José Hélio; Fernandes de Melo, Dirce; Gouveia, Zélia; Cardoso, Hélia Guerra; Peixe, Augusto; Arnholdt-Schmitt, BirgitGenomic design' refers to the structural organization of gene sequences. Recently, the role of intron sequences for gene regulation is being better understood. Further, introns possess high rates of polymorphism that are considered as the major source for speciation. In molecular breeding, the length of gene-specific introns is recognized as a tool to discriminate genotypes with diverse traits of agronomic inte...
Differential expression and co-regulation of carrot AOX genes (Daucus carota)
Campos, Maria Doroteia; Cardoso, Hélia Guerra; Linke, Bettina; Costa, José Hélio; Fernandes de Melo, Dirce; Justo, Lígia; Frederico, António MiguelAlternative oxidase (AOX) is a mitochondrial protein encoded by the nuclear genome. In higher plants AOX genes form a small multigene family mostly consisting of the two subfamilies AOX1 and AOX2. Daucus carota L. is characterized by a unique extension pattern of AOX genes. Different from other plant species studied so far it contains two genes in both subfamilies. Therefore, carrot was recently highlighted as ...
Isolation of total RNA from ripe and unripe soursop (Annona muricata L.) fruit
Brasil, Isabella Montenegro; Otoch, Maria de Lourdes Oliveira; Costa, José Hélio; Maia, Geraldo Arraes; Lima, Maria da Guia SilvaSoursop fruit tissue is known by its acidic pH and high levels of polysaccharides, polyphenolics and secondary metabolites. These conditions are recognized to interfere unfavorably with conventional methodologies for RNA isolation. We describe here a rapid and simple method for the isolation of total RNA from soursop fruit. RNA was extracted in less than 4 h through a combination of SDS/potassium acetate precip...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||