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Adhesion of adipose-derived mesenchymal stem cells to patterned protein-glycosa...
Costa, Diana Soares da; Márquez-Posadas, M. C.; Araújo, A. R.; Yang, Y.; Becher, J.; Schnabelrauch, M.; Merino, S.; Groth, T.; Reis, R. L.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; Proteins and glycosaminoglycans (GAGs) are the main constituents of the extracellular matrix (ECM). Until very recently, GAGs were considered as pure structural components while proteins have been associated with crucial cell signalling processes. Nowadays, it is well accepted that these two ECM components act in s...
Sulfation degree of glycosaminoglycans triggers distinct cytoskeleton organisat...
Costa, Diana Soares da; Yang, Y.; Moller, Stephanie; Groth, T.; Reis, R. L.; Pashkuleva, I.Glycosaminoglycans (GAGs) comprise the closest cellular environment: they are building elements of the ECM and can be also found on cells surface. Their biological activity depends on several parameters among which the negative charge is of prime importance[1]. This charge is generally associated with the presence of sulfate groups (-OSO3H). Sulfation is a dynamic modification: it can occur at various positions...
Sulfonic groups induce formation of filopodia in mesenchymal stem cells
Costa, Diana Soares da; Pires, R. A.; Frias, A. M.; Reis, R. L.; Pashkuleva, I.Glycosaminoglycans (GAGs) are an integral part of the extracellular matrix and glycocalix, i.e. the closest cellular environment. They are abundant in –OH groups and their bioactivity is also associated with the presence of negatively charged –SO3H functionalities. Therefore, we have investigated and discussed the influence of these functional units on mesenchymal stem cells (MSCs) behaviour using single compon...
GAGs-thiolated chitosan assemblies for chronic wounds treatment : control of en...
Francesko, Antonio; Costa, Diana Soares da; Lisboa, P.; Reis, R. L.; Pashkuleva, I.; Tzanov, TzankoMultilayered polyelectrolyte coatings comprising thiolated chitosan (TC) and glycosaminoglycans (GAGs), chondroitin sulphate and hyaluronic acid, were built using a layer-by-layer approach. The surface activity of these coatings for binding and inhibition of enzymes related to chronic inflammation, such as collagenase and myeloperoxidase, was assessed. The build-up of five bi-layers of TC/GAGs onto gold surface...
Thiolated chitosan/glycosaminoglycans multilayered films : QCM-D study on the f...
Francesko, Antonio; Díaz-Blanco, Carlos; Lisboa, P.; Costa, Diana Soares da; Pashkuleva, I.; Reis, R. L.; Tzanov, TzankoLayer-by-layer technique is widely used to produce polyelectrolyte multilayered films for material surface functionalization. The technique simplicity coupled with the biological potential of biopolymers, e.g. polysaccharides, make such assemblies a suitable choice for many biomedical applications. In this study the formation of the films comprising of thiolated chitosan and glycosaminoglycans (GAGs) by alterna...
Cardosins in postembryonic development of cardoon: towards an elucidation of th...
Pereira, Cláudia Sofia; Costa, Diana Soares da; Pereira, Susana; Nogueira, F. de Moura; Albuquerque, P. M.; Teixeira, J.; Faro, C.; Pissarra, J.Summary. Following on from previous work, the temporal and spatial accumulation of the aspartic proteinases (EC 3.4.23) cardosin A and cardosin B during postembryonic seed development of cardoon (Cynara cardunculus) was studied. mRNA and protein analyses of both cardosins suggested that the proteins accumulate during seed maturation, and that cardosin A is later synthesised de novo at the time of radicle emerg...
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