The diversity of yeast species related with the grape variety Touriga Nacional was evaluated at four distinct vineyards of three Portuguese wine regions. Samples were collected 48 h after crushing, and when the loss of CO2 reached 70 g/L. Restriction profiles analysis of 26S rDNA region with the endonucleases HaeIII, CfoI, HinfI, ApaI and MseI was used to characterize the five hundred and nine isolates obtained...
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