Encontrados 22 documentos, a visualizar página 1 de 3
Analysis of pancreas histological images for glucose intolerance identification...
Rato, L.M.; Capela e Silva, F.; Costa, A.R.; Antunes, C.M.The observation in microscopy of histological sections allows us to evaluate structural differences, in pancreatic cells, between rats with normal glucose tolerance and with glucose intolerance (pre-diabetic) situation. Nevertheless, this pre-diabetic condition implies subtle changes in islets of Langerhans structure. This and the normal variability among sampled cells makes difficult the task of identifying gl...
Influence of taste sensitivity in diet choices and obesity among children.
Lamy, E.; Pinheiro, C.; Costa, A.R.; Rodrigues, L; Antunes, CMIntroduction: The prevalence of childhood overweight and obesity in Portugal is about 35% (Rito et al., 2012). Lifestyle and dietary habits are important risk factors for weight gain. Food choice and acceptance greatly relies on food perception. The evaluation of taste sensitivity in children and its relation to dietary choices and Body Mass Index (BMI) will be important in a context of obesity prevention. None...
Expression and characterization of a therapeutic monoclonal antibody in mammali...
Costa, A. R.Doctoral dissertation for PhD degree in Biomedical Engineering ; The advent of therapeutic recombinant proteins has revolutionized modern medicine. Since the approval of recombinant insulin in 1982 to treat diabetes, many other recombinant proteins have emerged for a diversity of previously incurable conditions. In these years, the manufacturing processes have greatly evolved, but have also often disregarded p...
The impact of microcarrier culture optimization on the glycosylation profile of...
Costa, A. R.; Withers, Joanne; Rodrigues, E.; McLoughlin, Niaobh; Henriques, Mariana; Oliveira, Rosário; Rudd, Pauline M.; Azeredo, JoanaMicrocarriers are widely used for the large-scale culture of attachment-dependent cells with increased cell densities and, ultimately, higher product yield. In these processes, the specific culture conditions can affect the quality of the product, which is closely related to its glycosylation pattern. Furthermore, the lack of studies in the area reinforces the need to better understand the effects of microcarri...
Advances and drawbacks of the adaptation to serum-free culture of CHO-K1 cells ...
Rodrigues, E.; Costa, A. R.; Henriques, Mariana; Cunnah, Philip; Melton, David; Azeredo, Joana; Oliveira, RosárioCurrently, mammalian cell technology has become the focus of biopharmaceutical production, with strict regulatory scrutiny of the techniques employed. Major concerns about the presence of animal-derived components in the culture media led to the development of serum-free (SF) culture processes. However, cell adaptation to SF conditions is still a major challenge and limiting step of process development. Thus, t...
Ehanced oil recovery under simulated reservoir conditions using an indigenous B...
Gudiña, Eduardo J.; Pereira, Jorge F. B.; Costa, A. R.; Rodrigues, L. R.; Coutinho, J. A. P.; Teixeira, J. A.Microbial Enhanced Oil Recovery (MEOR) is potentially useful to increment oil recovery from reservoirs beyond primary and secondary recovery operations using microorganisms and their metabolites. In situ stimulation of microorganisms that produce biosurfactants and degrade heavy oil fractions reduces the capillary forces that retain the oil inside the reservoir and decreases oil viscosity, thus promoting its fl...
Production and characterization of biosurfactants produced by microorganisms is...
Pereira, Jorge F. B.; Costa, A. R.; Gudiña, Eduardo J.; Rodrigues, L. R.; Teixeira, J. A.; Coutinho, J. A. P.Surfactants are amphiphilic molecules that comprise both hydrophilic and hydrophobic moieties, allowing the reduction the surface and interfacial tensions, as well as the formation of oil in water or water in oil emulsions. These surface-active compounds are extensively used by petroleum industries in order to reduce the capillary forces that entrapped the oil inside the reservoir. The compounds synthetized che...
Variations in salivary function in a rodent model of pre-diabetes
Rodrigues, Lénia; Mouta, R.; Barra, C.; Costa, A. R.; Silva, F. C. e; Lopes, O.; Lima, M.; Antunes, C.; Lamy, E.Diabetes is a widespread disease representing an enormous part of the total health costs. An early diagnostic could be of extremely importance both for the understanding and prevention of this pathology. Saliva is a fluid with increasing interest as a source of biomarkers for disease diagnostic and saliva protein composition changes have already been reported for diabetic individuals. However, the studies were ...
Preliminary evaluation of microcarrier culture for growth and monoclonal antibo...
Rodrigues, E.; Fernandes, Pedro; Costa, A. R.; Henriques, Mariana; Azeredo, Joana; Oliveira, RosárioLarge-scale biopharmaceutical production commonly relies on suspension cell cultures that provide higher yields than adherent cultures. However, most mammalian cells grow adherently and therefore need to be adapted to suspended growth, which is not always simple or feasible. Microcarrier culture introduces new possibilities and makes achievable the practical high yield culture of anchorage-dependent cells in su...
Strategies for adaptation of mAb-producing CHO cells to serum-free medium
Costa, A. R.; Rodrigues, E.; Henriques, Mariana; Oliveira, Rosário; Azeredo, JoanaLarge-scale production of biopharmaceuticals commonly requires the use of serum-free medium, for safety and cost reasons. However, serum is essential to most mammalian cells growth, and its removal implies a very time-consuming process for cell adaptation. Thus, the aim of the study was to evaluate different strategies for cell adaptation to serum-free medium. Three cell types were used to assess the impact of...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||