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Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from ...
Cardoso, L.; Cortes, H.C.E.; Reis, A.; Rodrigues, P.; Simões, M.; Lopes, A.P.; Vila-Viçosa, M.J.; Talmi-Frank, D.; Eyal, O.; Solano-Gallego, L.The prevalence of piroplasm (order Piroplasmida) infection was assessed in blood and bone marrow samples from 91 red foxes (Vulpes vulpes) from northern, central and southern Portugal by means of molecular methods. PCR for the 18S rRNA gene of Babesia spp. followed by sequencing revealed 63 foxes positive for the Babesia microti-like piroplasm (syn. Theileria annae) (69.2%; 95% confidence interval [CI]: 58.7-78...
Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from ...
Cardoso, L.; Cortes, H.C.E.; Reis, A.; Rodrigues, P.; Simões, M.; Lopes, A.P.; Vila-Viçosa, M.J.; Talmi-Frank, D.; Eyal, O.; Solano-Gallego, L.Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from Portugal Abstract The prevalence of piroplasm (order Piroplasmida) infection was assessed in blood and bone marrow samples from 91 red foxes (Vulpes vulpes) from northern, central and southern Portugal by means of molecular methods. PCR for the 18S rRNA gene of Babesia spp. followed by sequencing revealed 63 foxes positive for the...
Mini-FLOTAC for the diagnosis of Eimeria infection in goats: An alternative to ...
Silva, L.M.R.; Vila-Viçosa, M.J.M.; Maurelli, M.P.; Morgoglione, M.E.; Cortes, H.C.E.; Cringoli, G.; Rinaldi, L.Caprine coccidiosis is responsible for remarkable economic losses. Diagnosis must therefore take into account a number of epidemiological and clinical factors supported by laboratory investigations. The detection of Eimeria oocysts and the determination of oocysts per gram of faeces (OPG) require a trustworthy oocyst count technique. Mini-FLOTAC is a new apparatus from the FLOTAC family, which was designed for ...
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle...
Schares, G.; Basso, W.; Majzoub, M.; Cortes, H.C.E.; Rostaher, A.; Selmair, J.; Hermanns, W.; Conraths, F.J.; Gollnick, N.S.Besnoitia besnoiti was in vitro isolated during the first recorded outbreak of bovine besnoitiosis in Germany. Molecular characterization of the new isolate, named Bb-GER1, revealed almost 100% identity with other B. besnoiti isolates obtained in Portugal, Spain, Israel or South Africa, when partial sequences of the 18S ribosomal RNA gene, of the internal transcribed spacer 1 and of the 5.8S RNA gene were compa...
Rastreos del grupo de lucha contra la equinococosis-hidatidosis en los municipi...
HERNANDEZMIRA, G; Cortes, H.C.E.; Pacheco, A.; Fontes, I.The activity of this multidisciplinary Group is running in north-east Alentejo, an area where there are high prevalence level of parasitism by Echinococcus granulosus. This factor, lead to the first of survey in 1991 and the establishment of a multidisciplinary group fighting Hydatidosis- Echinococcosis on a regular basis in 1998. The group develops different activities: Surveys in humans (epidemiological quest...
In vitro efficacy of nitro- and bromo-thiazolyl-salicylamide compounds (thiazol...
CORTES, H. C. E.; MUELLER, N.; ESPOSITO, M.; LEITÃO, A.; NAGULESWARAN, A.; HEMPHILL, A.Nitazoxanide (NTZ) and its deacetylated metabolite tizoxanide (TIZ) exhibit considerable in vitro activity against Besnoitia besnoiti tachyzoites grown in Vero cells. Real-time-PCR was used to assess B. besnoiti tachyzoite adhesion, invasion, and intracellular proliferation in vitro. A number of NTZ-derivatives, including Rm4822 and Rm4803, were generated, in which the thiazole-ring-associated nitro-group was r...
Isolation of Besnoitia besnoiti from infected cattle in Portugal
Cortes, H.C.E.; Reis, Y.; Waap, H.; Vidal, R.; Soares, H.; Marques, I.; Pereira da Fonseca, I.; Fazendeiro, I.; Ferreira, M.L.; Caeiro, V.; Shkap, V.Abstract Besnoitia besnoiti, an obligate intracellular protozoan parasite belonging to the phylum apicomplexa, is the causative agent of bovine besnoitiosis. Besnoitiosis is responsible for significant losses in the cattle industry of Africa and Mediterranean countries due to the high morbidity rate, abortion and infertility in males. The acute stage of disease is associated with the proliferative forms (tachyz...
Immunodiagnosis of Besnoitia besnoiti infection by ELISA and Western blot
Cortes, H.C.E.; Nunes, S.; Reis, Y.; Staubli, D.; Vidal, R.; Sager, H.; Leitão, A.; Gottstein, B.Abstract Besnoitia besnoiti, an obligate intracellular apicomplexan protozoan parasite, is the causative agent of bovine besnoitiosis. This infection may dramatically affect body condition and lead to irreversible infertility in males, resulting in important economical losses in livestock production. Identification of serologically positive animals is of major relevance to elaborate appropriate measures of cont...
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