Encontrados 12 documentos, a visualizar página 1 de 2
Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from ...
Cardoso, L.; Cortes, H.C.E.; Reis, A.; Rodrigues, P.; Simões, M.; Lopes, A.P.; Vila-Viçosa, M.J.; Talmi-Frank, D.; Eyal, O.; Solano-Gallego, L.The prevalence of piroplasm (order Piroplasmida) infection was assessed in blood and bone marrow samples from 91 red foxes (Vulpes vulpes) from northern, central and southern Portugal by means of molecular methods. PCR for the 18S rRNA gene of Babesia spp. followed by sequencing revealed 63 foxes positive for the Babesia microti-like piroplasm (syn. Theileria annae) (69.2%; 95% confidence interval [CI]: 58.7-78...
Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from ...
Cardoso, L.; Cortes, H.C.E.; Reis, A.; Rodrigues, P.; Simões, M.; Lopes, A.P.; Vila-Viçosa, M.J.; Talmi-Frank, D.; Eyal, O.; Solano-Gallego, L.Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from Portugal Abstract The prevalence of piroplasm (order Piroplasmida) infection was assessed in blood and bone marrow samples from 91 red foxes (Vulpes vulpes) from northern, central and southern Portugal by means of molecular methods. PCR for the 18S rRNA gene of Babesia spp. followed by sequencing revealed 63 foxes positive for the...
Mini-FLOTAC for the diagnosis of Eimeria infection in goats: An alternative to ...
Silva, L.M.R.; Vila-Viçosa, M.J.M.; Maurelli, M.P.; Morgoglione, M.E.; Cortes, H.C.E.; Cringoli, G.; Rinaldi, L.Caprine coccidiosis is responsible for remarkable economic losses. Diagnosis must therefore take into account a number of epidemiological and clinical factors supported by laboratory investigations. The detection of Eimeria oocysts and the determination of oocysts per gram of faeces (OPG) require a trustworthy oocyst count technique. Mini-FLOTAC is a new apparatus from the FLOTAC family, which was designed for ...
Besnoitia besnoiti protein disulfide isomerase (BbPDI): molecular characterizat...
Marcelino, E; Martins, TM; Morais, JB; Nolasco, S; Cortes, H; Hemphill, A; Leitão, A; Novo, CExp Parasitol. 2011 Oct;129(2):164-74. Epub 2011 Jul 3. Besnoitia besnoiti protein disulfide isomerase (BbPDI): molecular characterization, expression and in silico modelling. Marcelino E, Martins TM, Morais JB, Nolasco S, Cortes H, Hemphill A, Leitão A, Novo C. Source Instituto de Investigação Cientifica Tropical, CIISA, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Av. da Universidade Té...
A modified agglutination test for the diagnosis of Besnoitia besnoiti infection
Waap, H; Cardoso, R; Marcelino, E; Malta, J; Cortes, H; Leitão, AAbstract Bovine besnoitiosis is caused by Besnoitia besnoiti, an obligate intracellular apicomplexan parasite. Affected animals present cutaneous and systemic manifestations and the disease may lead to considerable economic losses. Although generally associated to tropical and subtropical areas, bovine besnoitiosis is now considered an emergent disease in Europe, due to the increasing number of new cases and a...
Comparative evaluation of immunofluorescent antibody and new immunoblot tests f...
Schares, G.; Basso, W.; Majzoub, M.; Rostaher, A.; Scharr, J.C.; Langenmayer, M.C.; Selmair, J.; Dubey, J.P.; Cortes, H.C.; Conraths, F.J.Besnoitia besnoiti, an economically important disease in cattle in many countries of Africa and Asia is re-emerging in Europe. Serological identification of infected cattle is important because introduction of these animals into naive herds seems to play a major role in the transmission of the parasite. We report new, simplified immunoblot-based serological tests for the detection of B. besnoiti specific antibo...
First in vitro isolation of Besnoitia besnoiti from chronically infected cattle...
Schares, G.; Basso, W.; Majzoub, M.; Cortes, H.C.E.; Rostaher, A.; Selmair, J.; Hermanns, W.; Conraths, F.J.; Gollnick, N.S.Besnoitia besnoiti was in vitro isolated during the first recorded outbreak of bovine besnoitiosis in Germany. Molecular characterization of the new isolate, named Bb-GER1, revealed almost 100% identity with other B. besnoiti isolates obtained in Portugal, Spain, Israel or South Africa, when partial sequences of the 18S ribosomal RNA gene, of the internal transcribed spacer 1 and of the 5.8S RNA gene were compa...
Rastreos del grupo de lucha contra la equinococosis-hidatidosis en los municipi...
HERNANDEZMIRA, G; Cortes, H.C.E.; Pacheco, A.; Fontes, I.The activity of this multidisciplinary Group is running in north-east Alentejo, an area where there are high prevalence level of parasitism by Echinococcus granulosus. This factor, lead to the first of survey in 1991 and the establishment of a multidisciplinary group fighting Hydatidosis- Echinococcosis on a regular basis in 1998. The group develops different activities: Surveys in humans (epidemiological quest...
In vitro efficacy of nitro- and bromo-thiazolyl-salicylamide compounds (thiazol...
CORTES, H. C. E.; MUELLER, N.; ESPOSITO, M.; LEITÃO, A.; NAGULESWARAN, A.; HEMPHILL, A.Nitazoxanide (NTZ) and its deacetylated metabolite tizoxanide (TIZ) exhibit considerable in vitro activity against Besnoitia besnoiti tachyzoites grown in Vero cells. Real-time-PCR was used to assess B. besnoiti tachyzoite adhesion, invasion, and intracellular proliferation in vitro. A number of NTZ-derivatives, including Rm4822 and Rm4803, were generated, in which the thiazole-ring-associated nitro-group was r...
Microtubule cytoskeleton behavior in the initial steps of host cell invasion by...
REIS, Y; CORTES, H; VISEUMELO, L; FAZENDEIRO, I; LEITAO, A; SOARES, H; Cortes, HMicrotubule cytoskeleton behavior in the initial steps of host cell invasion by Besnoitia besnoiti Besnoitia besnoiti is a protozoan parasite responsible for bovine besnoitiosis. Indirect immunofluorescence showed that isolated B. besnoiti possesses a set of subpellicular microtubules, radiating from the apical end and extending for more than 2/3 of the cell body. Upon interaction with the host cell, B. besnoi...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||