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Protein purification by affinity chromatography
Coelho, L. C. B. B.; Santos, Andréa F. S.; Napoleão, Thiago H.; Correia, M. T. S.; Paiva, Patrícia M. G.
Optimized extraction of a lectin from Crataeva tapia bark using AOT in isooctan...
Nascimento, Cynthia O.; Costa, Romero M. P. B.; Araújo, Regina M. S.; Chaves, Maria E. C.; Coelho, L. C. B. B.; Paiva, Patrícia M. G.; Teixeira, J. A.Crataeva tapia bark lectin was extracted from a crude extract into a reversed micelle phase of the anionic surfactant AOT in isooctane and back-extracted, to a final aqueous phase by addition of butanol. The effects of pH, ionic strength and surfactant concentration on the protein transfer process from the aqueous to the organic phase were characterized, being the best results obtained after 5 min of contact, u...
Purification of a lectin from Eugenia uniflora L. seeds and its potential antib...
Oliveira, M. D. L.; Andrade, C. A. S.; Magalhães, N. S.; Coelho, L. C. B. B.; Teixeira, J. A.; Cunha, M. G. C.; Correia, M. T. S.Aims: The aim of this work was to analyse the antimicrobial properties of a purified lectin from Eugenia uniflora L. seeds. Methods and Results: The E. uniflora lectin (EuniSL) was isolated from the seed extract and purified by ion-exchange chromatography in DEAE-Sephadex with a purification factor of 11·68. The purified lectin showed a single band on denaturing electrophoresis, with a molecular mass of 67 kDa...
Effect of different cultivation conditions on Trametes versicolor : morphologic...
Xavier, A. L.; Correia, M. T. S.; Amaral, A. L.; Ferreira, E. C.
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