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1,3-Propanediol production in a two-step process fermentation from renewable fe...
Mendes, Filipa Soares; González-Pajuelo, Maria; Cordier, Hélène; François, Jean M.; Vasconcelos, IsabelIn this work, the production of 1,3-propanediol from glucose and molasses was studied in a two-step process using two recombinant microorganisms. The first step of the process is the conversion of glucose or other sugar into glycerol by the metabolic engineered Saccharomyces cerevisiae strain HC42 adapted to high (>200 g l−1) glucose concentrations. The second step, carried out in the same bioreactor, was perfo...
A metabolic and genomic study of engineered saccharomyces cerevisiae strains fo...
Cordier, Hélène; Filipa, Mendes; Isabel, Vasconcelos; François, Jean M.Towards a global objective to producechemical derivatives by microbial processes, this work dealt with a metabolic engineering of theyeast Saccharomyces cerevisiae for glycerol production. To accomplish this goal, overexpression of GPD1was introduced in a tpi1D mutant defective in triose phosphateisomerase. This strategy alleviated the inositol-less phenotype of this mutant, by reducing the levelsof dihydroxyac...
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