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Measuring Retinal Fluorescein Leakage into the Human Vitreous with a Modified C...
Rui C. Bernardes; Conceição L. Lobo; José G. Cunha-VazA method is described to quantify blood-retinal barrier permeability to fluorescein. Data are collected from the vitreous of the human eye at two elapsed time intervals after intravenous fluorescein administration, using a modified confocal scanning laser ophthalmoscope. Data processing includes fundus image enhancement, image alignment, profile construction, filtering and interpol...
Proteomics as a tool to develop molecular indicators of nutritional condition i...
Rodrigues, Pedro; Richard, N.; de Vareilles, M.; Silva, Tomé S.; Conceição, L. E. C.Gilthead seabream is the main aquaculture species produced in Portugal, as well as in the Mediterranean. However, the winter disease syndrome, a series of metabolic and immune system disorders caused by low temperatures, affects seabream culture, leading to important industrial losses, either as mortalities or growth decreases during spring. These effects can be minimized through dietary optimisation, either im...
Neuregulin1/ErbB system: importance in the control of cardiovascular function
Lopes-Conceição, L; Dias-Neto, M; Fontes-Sousa, AP; Mendes-Ferreira, P; Maia-Rocha, C; Henriques-Coelho, T; de Keulenaer, G; Leite-Moreira, AFThe family of Neuregulins (NRG), growth factors like epidermal growth factor, is known to induce growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells. This family comprises four members, being NRG1 the most largely studied, particularly at the cardiovascular level. The biological effects of NRG1 in the adult heart are mediated by the tyrosine kinase receptors ErbB. In the adult h...
Evaluation of tear production with modified Schirmer tear test-1 during the neo...
Conceição,L.F.; Ribeiro,A.P.; Torres,M.L.M.; Lourenço,M.L.G.; Laus,J.L.The study aimed to evaluate tear production by means of modified Schirmer tear tes-1 (mSTT-1) in neonate cats. Likewise, correlation between mSTT-1 and STT-1 was assessed in vitro. Standard SST strips were cut in half and after eye lid opening, tear production of neonates (n=15) was daily measured in both eyes (mSTT-1), until the 7th day, and at day 14, 21, and 28. Animals were daily weighted until 28 days of a...
Skeletal quality in Senegalese sole: characterization of fish resulting from en...
Gavaia, Paulo J.; Dionísio, G.; Cardeira, J.; Cunha, M. E.; Pousão-Ferreira, P.; Conceição, L. E. C.; Cancela, LeonorThe Senegalese sole (Solea senegalensis) is characteristic from southern Europe and Mediterranean. It has recently been adapted for aquaculture production since it is well accepted by consumers and reaches high commercial values. After the initial description of the ontogenic events of skeletogenesis, systematic evaluation of the incidence of skeletal malformations by several groups revealed high levels of skel...
Nutrition, quality and performance in marine fish larvae
Conceição, L. E. C.; Alves-Martins, D.; Engrola, S.; Gavaia, Paulo J.; Richard, N.; Aragão, C.O cultivo de larvas de peixes marinhos é caracterizado por altas mortalidades e por problemas de qualidade, nomeadamente malformações esqueléticas. A solução de parte destes problemas passa por uma melhor compreensão dos requisitos nutricionais. Este trabalho pretende fazer uma revisão de trabalhos recentes que demonstram como os aminoácidos, os ácidos gordos e a vitamina K, mas também o regime alimentar, podem...
Daily oxygen consumption rhythms of Senegalese sole solea senegalensis (Kaup, 1...
Castanheira, F.; Martins, C. I. M.; Engrola, S.; Conceição, L. E. C.Senegalese sole is known to be a species with pronounced nocturnal feeding behaviour. However, as for most fish species, there is a lack of knowledge concerning the influence of such biological rhythmon metabolic rate. The aim of this study was to determine whether individual variation in routine and fed metabolic rate was affected by daily light–dark rhythms in juveniles of Senegalese sole. The individual oxyg...
Individual differences in metabolism predict coping styles in fish
Martins, C. I. M.; Castanheira, F.; Engrola, S.; Costas, B.; Conceição, L. E. C.Studies on metabolism usually rely on measurements of oxygen consumption obtained in respirometry chambers. Despite rigorous standardization there is still considerable interindividual variation in metabolic rates which is often ignored. Furthermore, housing in respirometry chambers implies handling and exposure to a new environment, conditions known to be a source of stress in animals. The extent of stress res...
Modulation of myocardial stiffness by beta-adrenergic stimulation - its role in...
Falcão-Pires, I.; Sousa, Adelino Fontes; Conceiçao, L. Lopes; Brás-Silva, Carmen; Leite-Moreira, AdelinoThe acute effects of beta-adrenergic stimulation on myocardial stiffness were evaluated. New-Zealand white rabbits were treated with saline (control group) or doxorubicin to induce heart failure (HF) (DOXO-HF group). Effects of isoprenaline (10(-10)-10(-5) M), a non-selective beta-adrenergic agonist, were tested in papillary muscles from both groups. In the control group, the effects of isoprenaline were also e...
Inclusion of protein hydrolysates in the diet of white seabream (Diplodus sargu...
Richard, N.; de Vareilles, M.; Gavaia, Paulo J.; Silva, Tomé S.; Cordeiro, O.; Yúfera, M.; Rodrigues, Pedro; Conceição, L. E. C.In order to investigate the effects of dietary protein hydrolysate on larval skeleton quality and larvae proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co-fed from first-feeding onwards with live feed and three microencapsulated diets ir differing only on the molecular weight of their protein hydrolysate fraction.
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