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Development of a new application of the comet assay to assess levels of O6-meth...

O6-methylguanine (O6meG) is one of the most premutagenic, precarcinogenic, and precytotoxic DNA lesions formed by alkylating agents. Repair of this DNA damage is achieved by the protein MGMT, which transfers the alkyl groups from the O6 position of guanine to a cysteine residue in its active center. Because O6meG repair by MGMT is a stoichiometric reaction that irreversibly inactivates MGMT, which is subsequent...

Supplementation of a western diet with golden kiwifruits (Actinidia chinensis v...

Twelve-gel slide format optimised for comet assay and fluorescent in situ hybri...

Variation in the measurement of DNA damage by comet assay measured by the ECVAG...

The comet assay has become a popular method for the assessment of DNA damage in biomonitoring studies and genetic toxicology. However, few studies have addressed the issue of the noted inter-laboratory variability of DNA damage measured by the comet assay. In this study, 12 laboratories analysed the level of DNA damage in monocyte-derived THP-1 cells by either visual classification or computer-aided image analy...

Protective effects of ursolic acid and luteolin against oxidative DNA damage in...

Consumption of fruits and vegetables is associated with a reduced risk of developing a wide range of cancers including colon cancer. In this study, we evaluated the effects of two compounds present in fruits and vegetables, ursolic acid, a triterpenoid, and luteolin, a flavonoid, on DNA protection and DNA repair in Caco-2 cells using the comet assay. Ursolic acid and luteolin showed a protective effect against ...

DNA base excision repair as a biomarker in molecular epidemiology studies

Comet assay-based methods for measuring DNA repair in vitro; estimates of inter...