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Image analysis application for the study of activated sludge floc size during t...

Mesquita, D. P.; Ribeiro, R. R.; Amaral, A. L.; Ferreira, E. C.; Coelho, M. A. Z.

Background, aim, and scope Fishery wastewater treatment can be compromised due to seasonal production. The use of sequencing batch reactors is not completely successful, despite flexibility being one of the principal advantages. Most research on activated sludge is performed using synthetic wastewater to ensure a stable and constant feed. The current work compared biomass morphology and settling ability using i...


Determination of stability in dressing salad lyophilized by digital image proce...

Silva, Kelly; Ferreira, E. C.; Amaral, A. L.; Leão, M. H. M. Rocha; Coelho, M. A. Z.

Digital image processing (DIP) is a powerful tool that can be used to describe the loss of emulsion stability by molecular diffusion or coalescence. The stability of salad dressings (lemon juice and extra virgin olive oil) microencapsulated with biopolymers (without sorbitol) was analyzed by fluorescence DIP for a 110 days period. The emulsions were stored both refrigerated (4ºC) and at room temperature (27ºC)....


Yarrowia lipolytica : an industrial workhorse

Coelho, M. A. Z.; Amaral, P. F. F.; Belo, Isabel

Yarrowia lipolytica is one of the most extensively studied ‘‘non-conventional’’ yeasts, being a strictly aerobic microorganism capable of producing important metabolites and having an intense secretory activity, which justifies efforts to use it in industry (as a biocatalyst), in molecular biology and in genetics studies. Moreover, Y. lipolytica has been considered an adequate model for dimorphism studies in ye...


Study of saline wastewater influence on activated sludge flocs through automate...

Mesquita, D. P.; Amaral, A. L.; Ferreira, E. C.; Coelho, M. A. Z.

BACKGROUND: In activated sludge systems, sludge settling ability is considered a critical step in effluent quality and determinant of solid-liquid separation processes. However, few studies have reported the influence of saline wastewater on activated sludge. This work aims the evaluation of settling ability properties of microbial aggregates in a sequencing batch reactor treating saline wastewaters of up to 60...


Stalked protozoa identification by image analysis and multivariable statistical...

Amaral, A. L.; Ginoris, Y. P.; Nicolau, Ana; Coelho, M. A. Z.; Ferreira, E. C.

Protozoa are considered good indicators of the treatment quality in activated sludge systems as they are sensitive to physical, chemical and operational processes. Therefore, it is possible to correlate the predominance of certain species or groups and several operational parameters of the plant. This work presents a semiautomatic image analysis procedure for the recognition of the stalked protozoa species most...


SBR performance for synthetic and fishery wastewater treatment

Mesquita, D. P.; Ribeiro, R. R.; Teixeira, D.; Ferreira, E. C.; Coelho, M. A. Z.

The evaluation of a SBR performance treating synthetic and fishery wastewater was performed. Without salt addition, COD and ammonia removal rates over than 50% were obtained, indicating the occurrence of the first nitrification step and COD removal. With 0.5% salt, removal rates lower than 60% were attained, for ammonia removal efficiency probably due to ionic strength changes and cellular plasmolysis leading t...


Yarrowia lipolytica lipase production enhanced by increased air pressure

Lopes, Marlene; Gomes, Nelma; Gonçalves, Cristiana; Coelho, M. A. Z.; Mota, M.; Belo, Isabel

To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. Methods and Results: Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultures at atmospheric pressure. No inhibition of cellular growth was observed by the increase of pressu...


Raw data pre-processing in the protozoa and metazoa identification by image ana...

Ginoris, Y. P.; Amaral, A. L.; Nicolau, Ana; Coelho, M. A. Z.; Ferreira, E. C.

Different protozoa and metazoa populations develop in the activated sludge wastewater treatment processes and are highly dependent on the operating conditions. In the current work the protozoa and metazoa groups and species most frequent in wastewater treatment plants were studied, mainly the flagellate, sarcodine, and ciliate protozoa as well as the rotifer, gastrotrichia, and oligotrichia metazoa. The work is...


Aplicação de técnicas de análise de imagem e de estatística multivariável no re...

Ginoris, Y. P.; Amaral, A. L.; Ferreira, E. C.; Coelho, M. A. Z.; Nicolau, Ana

Os protozoários e pequenos metazoários são organismos abundantes nos tanques de aeração das Estações de Tratamento de Efluentes (ETEs) por lodos ativados. A distribuição de espécies e sua abundância têm sido apontadas como indicadores da qualidade do tratamento fornecendo um instrumento útil para avaliar o desempenho destes sistemas. O presente trabalho teve como objetivo o desenvolvimento em ambiente Matlab de...


Analysis of the effects of hyperbaric gases on S. cerevisiae cell cycle through...

Coelho, M. A. Z.; Coutinho, J. A. P.; Ferreira, E. C.; Mota, M.; Belo, Isabel

The effects of hyperbaric gases on the cell cycle of Saccharomyces cerevisiae were studied in batch cultures under pressures between 0.1 and 0.6 MPa and different gas compositions (air, oxygen, nitrogen or carbon dioxide). Classification of S. cerevisiae cells based on their morphology stages was obtained using an automatic image analysis procedure. Information on the distribution of different sub-populations a...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia