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A possible approach for gel-based proteomic studies in recalcitrant woody plants
Sebastiana, Mónica; Figueiredo, Andreia; Monteiro, Filipa; Martins, Joana; Franco, Catarina; Coelho, Ana Varela; Vaz, Fátima; Simões, TâniaWoody plants are particularly difficult to investigate due to high phenolic, resin, and tannin contents and laborious sample preparation. In particular, protein isolation from woody plants for two-dimensional gel electrophoresis (2-DE) is challenging as secondary metabolites negatively interfere with protein extraction and separation. In this study, three protein extraction protocols, using TCA, phenol and etha...
The Effect of Tannins on Mediterranean Ruminant Ingestive Behavior: The Role of...
Lamy, Elsa; Rawel, Harshadrai; Schweigert, Florian J.; Capela e Silva, Fernando; Ferreira, Ana; Costa, Ana Rodrigues; Antunes, CéliaAbstract: Sheep, cattle and goat are domestic ruminants of significant economic interest in the Mediterranean region. Although sharing the same pasture ranges, they ingest different plants and plant parts and, consequently different levels of tannins. This suggests an ability to detect and adapt ingestion according to animal physiological limits of tolerance for plant secondary metabolites. This review will det...
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