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Chemical composition and antioxidant activity of sulphated polysaccharides extr...
Rodríguez-Jasso, Rosa María; Mussatto, Solange I.; Pastrana, Lorenzo; Aguilar, Cristóbal N.; Teixeira, J. A.Sulphated polysaccharides (SP) were extracted from Fucus vesiculosus seaweed by using two different hydrothermal processes: microwave-assisted extraction (MAE) and autohydrolysis (AH). The extraction yields, chemical composition, and antioxidant activity of the polysaccharides extracted were determined and compared. Although both processes afforded SP with similar yields (18.2 mass % and 16.5 mass %, for MAE an...
Application of a high-throughput process analytical technology metabolomics pip...
Castro, C. C.; Martins, Rui C.; Teixeira, J. A.; Ferreira, António C.Abstract Metabolomics aims at gathering the maximum amount of metabolic information for a total interpretation of biological systems. A process analytical technology pipeline, combining gas chromatography�mass spectrometry data preprocessing with multivariate analysis, was applied to a Port wine �forced ageing� process under different oxygen saturation regimes at 60 �C. It was found that extreme �forced ageing�...
An overview of the recent developments on fructooligosaccharide production and ...
Dominguez, Ana; Rodrigues, L. R.; Lima, Nelson; Teixeira, J. A.Over the past years, many researchers have suggested that deficiencies in the diet can lead to disease states and that some diseases can be avoided through an adequate intake of relevant dietary components. Recently, a great interest in dietary modulation of the human gut has been registered. Prebiotics, such as fructooligosaccharides (FOS), play a key role in the improvement of gut microbiota balance and in in...
Extraction of sulfated polysaccharides by autohydrolysis of brown seaweed Fucus...
Rodríguez-Jasso, Rosa María; Mussatto, Solange I.; Pastrana, Lorenzo; Aguilar, Cristóbal N.; Teixeira, J. A.The extraction of sulfated polysaccharides (fucoidan) by autohydrolysis (AH) of brown seaweed Fucus vesiculosus was studied. Experimental assays were performed under different conditions of temperature (160 to 200°C) and reaction time (10 to 30 min) according to a 22 central composite design, and the conditions able to maximize the fucoidan yield were selected. The alga degradation and the total sugar yield in ...
Continuous-flow precipitation of hydroxyapatite in ultrasonic microsystems
Castro, Filipa; Kuhn, Simon; Jensen, Klavs; Ferreira, António; Rocha, Fernando; Vicente, A. A.; Teixeira, J. A.This paper describes the continuous-flow precipitation of hydroxyapatite Ca5(PO4)3OH (HAp) in two ultrasonic microreactors using diluted aqueous solutions of calcium and phosphate at 37 °C. Precipitation of HAp was first carried out in a tubular microreactor immersed in an ultrasonic bath, where single-phase (laminar) flow and segmented gas-liquid flow were both evaluated. The single-phase flow study was then c...
Laccase recovery with aqueous two-phase systems : enzyme partitioning and stabi...
Silvério, Sara C.; Rodríguez, Oscar; Tavares, Ana P. M.; Teixeira, J. A.; Macedo, EugéniaIn this work, the potential of several aqueoustwo-phase systems (ATPS) for laccaserecovery was evaluated. For this purpose, different types of ATPS were prepared and the partition coefficient of pure commercial laccase was determined in each one, at 23 °C. A total of 21 ATPS were investigated: 12 composed by a polymer and a salt and 9 composed by two different polymers. For polymer-salt ATPS, different composit...
Biorefinery valorization of autohydrolysis wheat straw hemicellulose to be appl...
Ruiz, Héctor A.; Cerqueira, M. A.; Silva, Hélder D.; Rodríguez-Jasso, Rosa María; Vicente, A. A.; Teixeira, J. A.The aims of this study were the extraction of hemicellulose from wheat straw (WS) and its utilization in the reinforcement of a κ-carrageenan/locust bean gum (κ-car/LBG) polymeric blend films (PBFs). WS hemicellulose extraction was performed under autohydrolysis process and hemicellulose extracted (HE) under optimum condition was used in PBFs. PBFs were prepared varying different proportions of HE into the κ-ca...
Nanoencapsulation of bovine lactoferrin for food and biopharmaceutical applicat...
Balcão, V. M.; Costa, Carla Isabel; Matos, Carla M.; Moutinho, C.; Amorim, Manuela; Pintado, Manuela E.; Gomes, Ana P.; Vila, Marta M.; Teixeira, J. A.Lactoferrin has for long captured the interest of many researchers as a natural compound with a wide variety of uses. Lactoferrin is a monomeric, iron-binding 80 kDa glycoprotein, and appears to be the subfraction of whey with the best documented antiviral, antimicrobial, anticancer and immune modulating/enhancing effects. It belongs to the family of transferrin proteins, and serves to control iron levels in bo...
Utilization of galactomannan from Gleditsia triacanthos in polysaccharide-based...
Cerqueira, M. A.; Souza, B. W. S.; Teixeira, J. A.; Vicente, A. A.The objective of this work was to evaluate the effect of the concentrations of Gleditsia triacanthos galactomannan and glycerol and the presence of corn oil in the physical properties of edible films. The influence of interactions between those constituents on films' permeability to gases (water vapour, CO2 and O2), solubility in water, mechanical properties and colour was evaluated. The effects of those variab...
Maximization of fructooligosaccharides and β-fructofuranosidase production by A...
Mussatto, Solange I.; Ballesteros, Lina F.; Martins, Silvia; Maltos, Dulce A .F.; Aguilar, Cristóbal N.; Teixeira, J. A.The conditions of temperature, moisture content, and inoculum rate able to maximize the production of fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) enzyme by solid-state fermentation were established. Fermentation assays were performed using the support material (coffee silverskin) moistened to 60, 70, or 80 % with a 240-g/l sucrose solution and inoculated with a spore suspension of Aspergillus ...
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