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Characterization of contaminants from a sanitized milk processing plant

Milk processing lines offer a wide variety of microenvironments where a diversity of microorganisms can proliferate. We sampled crevices and junctions where, due to deficient reach by typical sanitizing procedures, bacteria can survive and establish biofilms. The sampling sites were the holding cell, cold storage tank, pasteurizer and storage tank - transfer pump junction. The culturable bacteria that were isol...

Mixing and matching siderophore clusters : structure and biosynthesis of serrat...

Studying the evolutionary history underlying the remarkable structures and biological activities of natural products has been complicated by not knowing the functions they have evolved to fulfill. Siderophores - soluble, low molecular weight compounds - have an easily understood and measured function: acquiring iron from the environment. Bacteria engage in a fierce competition for acquiring iron, which rewards ...

The effects of a biocide and a surfactant on the detachment of Pseudomonas fluo...

Application of antimicrobial chemicals is a general procedure in the cleaning and disinfection of food-contacting surfaces. Adhesion to glass surfaces and chemically induced detachment of Pseudomonas fluorescens ATCC 13525T were studied in situ, under flow conditions, in a well-controlled parallel plate flow chamber (PPFC). Ortho-phthalaldehyde (OPA) and cetyltrimethyl ammonium bromide (CTAB) were applied separ...

Serratia plymuthica dairy industry isolates and their antimicrobial metabolites...

Background and aims: Phenotypical differences from interstrain variability is a known phenomenon, assessed in this study for V4 and Y S. plymuthica isolates, particularly at antimicrobial metabolites production and effect on pathogens biofilms. Methods: Isolates were biochemically characterized, specific growth rates in Tryptic Soy/Skim Milk Broth determined, and the antimicrobial activity of cell-free spent me...

Bacillus cereus-Serratia plymuthica territorial interactions

Impact of antimicrobial metabolites produced by Serratia plymuthica dairy indus...

Production of siderophores and quorum sensing antagonists and free energy of ad...

Influence of biofilm composition on the resistance to detachment

Bacillus cereus and Pseudomonas fluorescens were used to develop monoculture biofilms in a bioreactor rotating system using a stainless steel cylinder for biofilm formation. The biofilms were allowed to grow for 7 days, exposed continuously to a Reynolds number of agitation (ReA) of 2,400. Afterwards, the biofilms were characterised in terms of respiratory activity, amount of biomass, cellular density, cellular...

Antimicrobial mechanisms of ortho-phthalaldehyde action

Biocides generally have multiple biochemical targets. Such a feature easily entangles the analysis of the mechanisms of antimicrobial action. In this study, the action of the dialdehyde biocide ortho-phtalaldehyde (OPA), on bacteria, was investigated using the Gram-negative Pseudomonas fluorescens. The targets of the biocide action were studied using different bacterial physiological indices. The respiratory ac...

Microbial interactions in biofilms : role of siderophores and iron-dependent me...

Biofilms are ubiquitous in nature and can cause significant problems in public health, medicine and industry. Antimicrobial approaches to treat bacterial proliferation and biofilm formation constitute a focal point of modern research. We are entering a post-chemical antimicrobial era, not only due to the need to delivering of environmentally- friendly products, but also due to the increasing resistance of some ...