Encontrados 14 documentos, a visualizar página 1 de 2
First characterization of infectious cDNA clones of Olive mild mosaic virus
Cardoso, Joana M.S.; Félix, M. Rosário; Clara, M. Ivone; Oliveira, SolangeFull-length cDNA clones of an Olive mild mosaic virus (OMMV) isolate were constructed in order to find infectious cDNA clones. The sequencing of three individual full-length clones revealed some differences between them. In vitro transcription of these clones was performed and the effect of spontaneous mutations in the biological behaviour of the in vitro transcripts was evaluated by symptomatology, RNA accumul...
Biology and molecular characterization of necroviruses
Félix, M. Rosário; Varanda, Carla M.R.; Clara, M. Ivone E.Three necroviruses, family Tombusviridae, are known to infect Olea europaea L., Olive latent virus 1 (OLV-1), Olive mild mosaic virus (OMMV) and Tobacco necrosis virus D (TNV-D), often asymptomatically. OLV-1 has a wide geographical distribution whereas that of OMMVand TNV-D is not known as their discovery is recent, dating from 2005 and 2009, respectively. OMMV has received special attention because of its ori...
Specific amino acids of Olive mild mosaic virus coat protein are involved in tr...
Varanda, Carla; Félix, Maria R.; Soares, Cláudio M.; Oliveira, Solange; Clara, M. IvoneAbstract: Transmission of Olive mild mosaic virus (OMMV) is facilitated by Olpidium brassicae (Wor.) Dang. An OMMV mutant (OMMVL11) containing two changes in the coat protein (CP), asparagine to tyrosine at position 189 and alanine to threonine at position 216, has been shown not to be Olpidium brassicae-transmissible owing to inefficient attachment of virions to zoospores. In this study, these amino acid chang...
Evidence of olive mild mosaic virus transmission by Olpidium brassicae
Varanda, Carla M. R.; Silva, Marta S. M. R.; Félix, M. Rosário; Clara, M. IvoneTransmission of three strains of OMMV by an Olpidium sp. was evaluated and compared. The three strains were 1) an OMMV wild type (WT) recovered from olive trees, 2) an OMMV variant (L11) obtained after 15 serial passages of single local lesions induced in Chenopodium murale plants, and 3) a construct OMMV/OMMVL11 in which the coat protein (CP) gene replaced that of the wild type. A single-sporangial culture der...
Multiplex RT-PCR for detection and identification of three necroviruses that in...
Varanda, Carla; Cardoso, Joana M.S.; Félix, M. Rosário; Oliveira, Solange; Clara, M.IvoneAn optimized multiplex RT-PCR assay was developed to discriminate three necrovirus (Olive latent virus 1 (OLV-1), Tobacco necrosis virus D (TNV-D) and Olive mild mosaic virus (OMMV)) that infect olive trees. An olive orchard consisting of 54 trees of cv. "Galega vulgar" in the south of Portugal was surveyed. dsRNA fraction was used as template and revealed the 3 viruses, singly or in multiple infections, presen...
Complete genome sequence of a Tobacco necrosis virus D isolate from olive trees
Cardoso, Joana S.; Félix, M.Rosário; Clara, M.Ivone; Oliveira, SolangeThe first complete genome sequence of an isolate of Tobacco necrosis virus D (TNV-D; genus Necrovirus,family Tombusviridae) was that of a bean isolate from England. The complete nucleotide sequence of another TNV-D isolate, an Hungarian isolate from tobacco plants (TNV-DH), revealed a similar genomic organization. The single-stranded positive-sense RNA genome of TNV-D is about 3.8 kb in size and contains six op...
Biological and Molecular Characterization of Olive latent virus 1
Félix, M. Rosário; Cardoso, Joana M.S.; Oliveira, Solange; Clara, M. Ivone E.Olive latent virus 1 (OLV-1) belongs to the Necrovirus genus, Tombusviridae family and is pathogenic to olive, citrus and tulip plants. It is easily mechanically transmissible to indicator plants causing necrotic lesions and can be transmitted through the soil into the plant roots in the absence of biological vectors. Infected cells contain virus aggregates, inclusions made up of excess of viral coded peptides ...
Biological and Molecular Characterization of Olive latent virus 1
Félix, M. Rosário; Cardoso, Joana M.S.; Oliveira, Solange; Clara, M. Ivone E.Olive latent virus 1 (OLV-1) belongs to the Necrovirus genus, Tombusviridae family and is pathogenic to olive, citrus and tulip plants. It is easily mechanically transmissible to indicator plants causing necrotic lesions and can be transmitted through the soil into the plant roots in the absence of biological vectors. Infected cells contain virus aggregates, inclusions made up of excess of viral coded peptides ...
Monoclonal antibody to Olive latent virus 1
Custódio, Ana I.; Cardoso, Fernando H.; Félix, M. Rosário; Clara, M. Ivone; Novo, CarlosOlive is a crop of high economic importance in Portugal. Its susceptibility to virus infections justifies research leading to accurate virus detection. Two necroviruses mechanically transmissible to herbaceous indicator plants have been isolated from symptomatic olive trees: Olive latent virus 1 (OLV-1) and Tobacco necrosis virus D (TNV-D). OLV-1 was also isolated from citrus trees in Turkey and from tulips in ...
The complete genome sequence of a new necrovirus isolated from Olea europaea L.
Cardoso, Joana M. S.; Félix, M. Rosário; Clara, M. Ivone; Oliveira, SolangeThe complete nucleotide sequence of a virus isolated from Olea europaea L. (GP isolate), previously identified as an isolate of Tobacco necrosis virus D (TNV-D) based on its coat protein sequence, was determined. The viral RNA genome consists of 3683 nucleotides and contains five open reading frames. The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity with that of an isolate of Olive laten...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||