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Scientifically advanced solutions for chestnut ink disease
Choupina, Altino; Estevinho, Leticia M.; Martins, IvoneOn the north regions of Portugal and Spain, the Castanea sativa Mill. culture is extremely important. The biggest productivity and yield break occurs due to the ink disease, the causal agent being the oomycete Phytophthora cinnamomi. This oomycete is also responsible for the decline of many other plant species in Europe and worldwide. P. cinnamomi and Phytophthora cambivora are considered, by the generality of ...
Cloning, characterization and in vitro and in planta expression of a glucanase ...
Martins, Ivone; Martins, Fátima; Belo, Hélio; Vaz, Madalena; Carvalho, Marisa; Cravador, Alfredo; Choupina, AltinoOomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. They are able to secrete a glucanase inhibitor protein (GIP) that inhibits the activity of endoglucanases (EGases) involved in defense responses against infection. One of the most widely distributed and aggressive Phytophthora species, with more than 1,000 host plants is P. cinnamom...
Developments in the fermentation process and quality improvement strategies for...
Iglesias, Antonio; Pascoal, Ananias; Choupina, Altino; Carvalho, Carlos Alfredo; Feás, Xesús; Estevinho, Leticia M.Mead is a traditional alcoholic drink derived from the fermentation of diluted honey in the presence of appropriate yeast. Its modern production, in general terms, involves the addition of nutrients to initial diluted honey, pasteurization, yeast inoculation, fermentation and removal of impurities. Undesirable events along the process have been reported; among them, we highlight: delayed or arrested fermentatio...
Transglutaminases: recent achievements and new sources
Martins, Ivone M.; Matos, Mauro; Costa, Rodrigo; Silva, Fátima; Pascoal, Ananias; Estevinho, Leticia M.; Choupina, AltinoTransglutaminases are a family of enzymes (EC 2.3.2.13), widely distributed in various organs, tissues, and body fluids, that catalyze the formation of a covalent bond between a free amine group and the γ-carboxamide group of protein or peptide-bound glutamine. Besides forming these bonds, that exhibit high resistance to proteolytic degradation, transglutaminases also form extensively cross-linked, generally in...
Análise e alinhamento de sequências biológicas
Choupina, Altino; Deusdado, SérgioO conhecimento derivado das tecnologias genómicas e computacionais aumenta em progressão geométrica. A compreensão dessa avalanche de dados está intimamente vinculada ao formidável desenvolvimento na área da bioinformática. Ao possibilitar a avaliação global dessa extraordinária quantidade de dados, a bioinformática tem acelerado consideravelmente as descobertas científicas. No software de análise do genoma pod...
Effect of temperature on the bioactive properties of bee pollen
Pascoal, A.; Rocha, J.F.; Choupina, Altino; Pereira, Ana Paula; Santos, Luís; Estevinho, Leticia M.Bee pollen is flower pollen collected by the honey bee, Apis mellifera, for the purpose of feeding its larvae in the early stages of development. It is recognized to be a valuable apitherapeutic product with potential for medical, health and nutritional applications. The objective of this work was to compares the effect of different storage conditions in the bioactive compounds and biological properties of bee ...
Efeito das condições de secagem na qualidade do pólen apícola
Rocha, Joana; Estevinho, Leticia M.; Choupina, AltinoO pólen apícola, com vastas aplicações na alimentação humana, resulta da aglutinação de grãos de pólen de várias fontes vegetais efetuada pelas abelhas, mediante acréscimo de substâncias salivares e pequenas quantidades de néctar. Este trabalho teve como objetivo comparar o efeito de diferentes condições de armazenamento nas características nutricionais e microbiológicas de pólen apícola biológico. Identificara...
Effect of storage conditions on the quality of the bee pollen
Estevinho, Leticia M.; Rocha, Joana; Pascoal, Ananias; Pereira, Ana Paula; Choupina, AltinoBee pollen (BP), is the result of the agglutination of flower pollens, made by worker honey bees, with nectar and salivary substances and collected at the hive entrance. The collection of AP is a relatively recent development, dependent primarily on the basic concept of scraping pollen of the bees' legs as they enter the hive. The objective of this work was to evaluate the effect of storage conditions in the ph...
Sequence analysis and expression of genes involved cytoskeleton and infection m...
Choupina, Altino; Estevinho, Leticia M.; Costa, Rodrigo; Dominguez, ÁngelOomycetes from the genus Phytophthora are plant pathogens that are devastating for agriculture and natural ecosystems. The biggest productivity and yield break occurs due to the ink disease; caused by Phytophthora cinnamomi, which is one of the most widely distributed Phytophthora species, with nearly 1 000 host species. The knowledge about the molecular mechanisms responsible for its pathogenicity is an import...
Enzimas β-Glucanases: aplicações da hidrólise de polissacarídeos /β-Glucanases ...
Costa, Rodrigo; Choupina, AltinoThe enzymatic degradation of polysaccharides is accomplished through the action of several hydrolases with different specificities and way of action. For this reason, there is now a growing interest in the study of these enzymes related to its potential industry. The β-glucanases enzymes belong to this group of hydrolases because of its ability to hydrolyze glycosidiclinkages β-D-glucosides 1,3-β-glucans and 1,...
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