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Influence of anaerobic conditions on vaginal microbiota recovery from bacterial...

Cereija, Tatiana; Castro, J.; Alves, P.; Cerca, Nuno

Bacterial vaginosis (BV) is one of the most common infections in women of reproductive age. Clinical studies have shown an association among BV and abnormal pregnancy, pelvic inflammatory disease and increased risk of sexually transmitted infections, including HIV. This disorder was first described in 1914 by Curtis as a “white discharge” syndrome and despite the decades of research we have only limited, and cl...


Isolation of Gardnerella vaginalis from BV patients and healthy women : analysi...

Castro, J.; Alves, P.; Sousa, Cármen; Cereija, Tatiana; Cerca, Nuno

Bacterial vaginosis (BV) is one of the most common gynaecological disorder affecting women in the reproductive age. Microbiological analysis of BV has shown Gardnerella vaginalis to be the most frequent organism in BV. However, G. vaginalis colonization do not always lead to BV. This raised the question whether there are pathogenic and commensal lineages within this species. In an effort to understand the diffe...


Gardnerella vaginalis virulence potential outcompetes with 30 other microorgani...

Alves, P.; Castro, J.; Sousa, Cármen; Cereija, Tatiana; Cerca, Nuno

Bacterial vaginosis (BV) is the most common vaginal disorder affecting millions of women every year, and is usually associated with several adverse health outcomes, including preterm birth and acquisition of sexually transmitted diseases. However, the etiology of BV is still under debate. Recently, new fastidious anaerobic bacteria have been associated with BV, but there are very few studies that comprehensivel...


Functional analysis of virulence potential from Gardnerella vaginalis and other...

Castro, J.; Machado, António; Alves, P.; Sousa, Cármen; Cereija, Tatiana; França, Ângela; Jefferson, Kimberly K.; Cerca, Nuno

In the past half century, bacterial vaginosis (BV) has been a controversial topic in medical microbiology, and despite the wealth of information on this topic, the etiological agent has not yet been definitively identified [1]. The first advances on BV pointed Gardnerella vaginalis as the infectious causative agent of BV [2] but soon after it was found that G. vaginalis was also present in healthy women [3]. Ad...


Lactobacilli and its metabolites as potential probiotics against Gardnerella va...

Cereija, Tatiana; Machado, A.; Felgueiras, C.; Cerca, Nuno; Rodrigues, L. R.

Bacterial vaginosis (BV) is one of the most common gynecological disorder affecting women in reproductive age. This condition poses a significant health risk because it predisposes women to abnormal pregnancy, pelvic inflammatory disease and an increased risk of sexual transmitted infections, including HIV [1]. Although BV etiology remains unknown, it is characterized by a decrease in vaginal lactobacilli and a...


Comparison of vaginal microbiota fingerprints from healthy and bacterial vagino...

Ferreira, Carla; Salvador, A. F.; Cereija, Tatiana; Cerca, Nuno; Sousa, D. Z.

Bacterial Vaginosis (BV) is a common disease in women of reproductive age and is characterized by the substitution of Lactobacillus species,which are predominant in the normal vaginal microbiota,by rapidly proliferating anaerobic bacteria, particularly Gardnerellavaginalis. The aim of this study was to study microbial communities’ structure in the vaginal microbiota of healthy and BV-positive Portuguese women. ...


In silico vs in vitro analysis of primer specificity for the detection of Gardn...

Henriques, Ana Filipa; Cereija, Tatiana; Machado, António; Cerca, Nuno

Bacterial vaginosis (BV) is a common pathology of women in reproductive age that can lead to serious health complications, and is associated with shifts in the normal microflora from predominance of Lactobacillus spp. to a proliferation of other anaerobes such as G. vaginalis and A vaginae, which can be detected by PCR. The optimal PCR pathogen detection assay relies mainly on the specificity and sensitivity of...


Characterization of biofilm-forming microorganisms isolated from vaginal exudat...

Alves, P.; Castro, J.; Cereija, Tatiana; Henriques, Ana Filipa; Cerca, Nuno

Bacterial vaginosis (BV) is one of the most common gynaecological conditions affecting women in the reproductive age, and can lead to increase risk gynaecological infections and pre-term labour. The aetiology of this pathology is still poorly understood, but recent reports referring to the presence of anaerobic biofilms both in the healthy and BV vagina (with different microbial compositions) have led to the th...


Characterization of trypsin inhibition by boophilin

Cereija, Tatiana Barros Pereira

No decurso da evolução, enquanto os vertebrados desenvolviam um complexo sistema para prevenir as perdas de sangue, os hematófagos criavam mecanismos anticoagulantes capazes de contra-atacar a reposta hemostática do hospedeiro, por forma a facilitar a extração, o armazenamento e a digestão do sangue. Muitos destes mecanismos assentam na inibição específica da trombina, proteína que tem um papel central na hemos...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia