Encontrados 7 documentos, a visualizar página 1 de 1
Amide conjugates of the DO3A-N-(alfa-amino)propionate ligand : leads for stable...
Ferreira, Miguel Filipe Moreira Marques; Martins, André F.; Martins, Catarina I. O.; Ferreira, Paula M. T.; Tóth, Éva; Rodrigues, Tiago B.A novel synthetic methodology for preparing amide conjugates of the DO3A-N-(alfa-amino)propionate chelator is described, using the synthesis of the DO3A-N-(alfa-benzoylamido)propionate chelator as an illustrative example. The model Gd(DO3A-N-(alfa-benzoylamido)propionate) chelate displays accelerated water exchange, stability in a wide pH range and inertness towards transmetallation by Zn2+. The Gd(DO3A-N-(al...
Gd(III)-EPTPAC16, a new self-assembling potential liver MRI contrast agent: in ...
Torres, Suzana; Prata, Maria I. M.; Santos, Ana C.; André, João P.; Martins, José A.; Helm, Lothar; Tóth, Éva; García-Martín, Maria L.The recently reported amphiphilic chelate, [Gd(EPTPAC16)(H2O)]2-, forms supramolecular aggregates in aqueous solution by self-assembly of the monomers with a relaxometrically determined critical micellar concentration (CMC) of 0.34 mM. The effect of sonication on the aggregate size was characterized by dynamic light scattering and relaxometry, indicating the presence of premicellar aggregates and an overall dec...
A method to measure lactate recycling in cultured cells by edited 1H nuclear ma...
Rodrigues, Tiago B.; Cerdán, Sebastián; García-Martín, María L.http://www.sciencedirect.com/science/article/B6W9V-4PF6B5M-1/1/385b6c0836057ee00a92ea234317f1e6
An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and ...
Rodríguez, Elisenda; Simoes, Rui; Roig, Anna; Molins, Elies; Nedelko, Nataliya; Slawska-Waniewska, Anna; Aime, Silvio; Arús, Carles; Cabañas, MiquelAbstract A new iron-based T 1 contrast agent consisting of a complex of iron ions coordinated to phosphate and amine ligands (Fe(phos) in short) has been characterized by spectroscopic and magnetic measurements. NMR relaxation studies showed r 1 values to be dependent on the phosphate salt concentration, K2HPO4, present in the medium. r 1 reaches a maximum value of 2.5 mM-1 s-1 for measurements carried out at ...
A fast and sensitive 1H NMR method to measure the turnover of the H2 hydrogen o...
Rodrigues, Tiago B.; Cerdán, SebastiánA fast and sensitive procedure to determine the turnover of the H2 hydrogen of lactate and quantify its 2H-enrichment by 1H NMR is illustrated using C6 cells metabolizing (3-13C) lactate in 50% 2H2O (vol/vol). 2H substitution of the lactate H2 hydrogen resulted in two easily detectable transformations of the vicinal H3 doublet resonance: 1) the formation of an H3 singlet due to the disappearance of the homonucl...
Futile cycling of lactate through the plasma membrane of C6 glioma cells as det...
Rodrigues, Tiago B.; Gray, Heather L.; Benito, Marina; Garrido, Susana; Sierra, Alejandra; Geraldes, Carlos F.; Ballesteros, Paloma; Cerdán, SebastiánWe report a novel (13C, 2H) nuclear magnetic resonance (NMR) procedure to investigate lactate recycling through the monocarboxylate transporter of the plasma membrane of cells in culture. C6 glioma cells were incubated with [3-13C]lactate in Krebs-Henseleit Buffer containing 50% 2H2O (vol/vol) for up to 30 hr. 13C NMR analysis of aliquots progressively taken from the medium, showed: (1) a linearly decreasing si...
13C MRS: An outstanding tool for metabolic studies
Rodrigues, Tiago B.; Cerdán, SebastiánWe provide an overview of 13C magnetic resonance spectroscopy methods and their applications as an established metabolic tool with an emphasis on the use of high-resolution 13C magnetic resonance spectroscopy and 13C isotopomer analysis. Topics addressed include general properties of the 13C magnetic resonance spectroscopy spectrum; different 13C magnetic resonance spectroscopy acquisition protocols; determinat...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||