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Sustained in vitro root development obtained in Pinus pinea inoculated with ect...
Oliveira, Paulo; Barriga, João; Cavaleiro, Cremilde; Peixe, Augusto; Zavattieri, AmelyStone pine (Pinus pinea L.) is an economically important forest tree in Mediterranean climates and has been the target for selection efforts through micropropagation. Previous attempts on microshoots, derived from mature seed cotyledons, reached incipient rooting after induction with a combination of auxin and hypertonic shock, but their development in vitro was not sustained. At this stage, co-culturing pla...
Gamma-irradiated pollen induces the formation of 2n endosperm and abnormal embr...
Peixe, Augusto; Campos, Maria; Cavaleiro, Cremilde; Barroso, João; Pais, MariaThe effect of gamma radiation on pollen germination capacity and pollen tube growth was evaluated in vitro and in situ conditions. In vitro experiments, revealed that irradiation significantly affects pollen viability, mainly for levels higher than 200 Grays (Gy). Also, for levels higher than 200 Gy, in situ observations showed that no pollen tube reached the ovule. Fruit set results confirmed that for irradiat...
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