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Evidence for diversifying selection in a set of Mycobacterium tuberculosis gene...
Osório, Nuno S.; Rodrigues, Fernando; Gagneux, Sebastien; Pedrosa, Jorge; Pinto-Carbó, Marta; Castro, António G.; Young, Douglas; Comas, IñakiThe authors acknowledge Margarida Correia-Neves and Catarina L. Santos for helpful comments and suggestions ; Tuberculosis (TB) is a global health problem estimated to kill 1.4 million people per year. Recent advances in the genomics of the causative agents of TB, bacteria known as the Mycobacterium tuberculosis complex (MTBC), have allowed a better comprehension of its population structure and provided the fo...
In vivo performance of chitosan/soy-based membranes as wound dressing devices f...
Santos, T. C.; Hoering, B.; Reise, Kathrin; Silva, S. S.; Oliveira, Joaquim M.; Mano, J. F.; Castro, António G.; Reis, R. L.; Griensven, Martijn vanWound management represents a major clinical challenge on what concerns healing enhancement and pain control. The selection of an appropriate dressing plays an important role in both recovery and esthetic ap- pearance of the regenerated tissue. Despite the wide range of available dressings, the progress in the wound care market relies on the increasing interest in using natural-based biomedical products. Herein...
VEGF and FGF-2 incorporation in starch-based bone tissue engineering constructs...
Santos, T. C.; Morton, Tatjana J.; Moritz, Martina; Pfeifer, Sabine; Reise, Kathrin; Marques, A. P.; Castro, António G.; Reis, R. L.The ideal bone tissue-engineered (TE) construct remains to be found, although daily discoveries significantly contribute to improvements in the field and certainly have valuable long-term outcomes. In this work, different TE elements, aiming at bone TE applications, were assembled and its effect on the expression of several vas- cularization/angiogenesis mediators analyzed. Starch/polycaprolactone (SPCL) scaffo...
Poor immune reconstitution in HIV-infected patients associates with high percen...
Horta, Ana; Nóbrega, Cláudia; Machado, Pedro Amorim; Teixeira, Vítor Emanuel Coutinho; Silva, Palmira Barreira; Boavida, Susana; Costa, Patrício SoaresCD4(+) regulatory T cells (Tregs) are essential for the maintenance of the immune system's equilibrium, by dampening the activation of potential auto-reactive T cells and avoiding excessive immune activation. To correctly perform their function, Tregs must be maintained at the right proportion with respect to effector T cells. Since this equilibrium is frequently disrupted in individuals infected with the human...
Mycobacterium tuberculosis strains are differentially recognized by TLRs with a...
Carmona, J. A.; Cruz, Andrea; Teixeira, Lucia de Fatima Moreira; Sousa, Carole Lara Veiga de; Sousa, Jeremy; Osório, Nuno S.; Saraiva, Ana L.Tuberculosis associates with a wide spectrum of disease outcomes. The Beijing (Bj) lineage of Mycobacterium tuberculosis (Mtb) is suggested to be more virulent than other Mtb lineages and prone to elicit non-protective immune responses. However, highly heterogeneous immune responses were reported upon infection of innate immune cells with Bj strains or stimulation with their glycolipids. Using both in vitro and...
Phage therapy is effective against infection by Mycobacterium ulcerans in a mur...
Trigo, Gabriela; Martins, Teresa G.; Fraga, Alexandra G.; Longatto Filho, Adhemar; Castro, António G.; Azeredo, Joana; Pedrosa, JorgeAuthor Summary : Buruli Ulcer (BU), caused by Mycobacterium ulcerans, is a necrotizing disease of the skin, subcutaneous tissue and bone. Standard treatment of BU patients consists of a combination of the antibiotics rifampicin and streptomycin for 8 weeks. However, in advanced stages of the disease, surgical resection of the destroyed skin is still required. The use of bacterial viruses (bacteriophages) for th...
The novel Fh8 and H fusion partners for soluble protein expression in Escherich...
Costa, Sofia Judite; Almeida, André; Castro, António G.; Domingues, Lucília; Besir, HüseyinThe Escherichia coli host system is an advantageous choice for simple and inexpensive recombinant protein production but it still presents bottlenecks at expressing soluble proteins from other organisms. Several efforts have been taken to overcome E. coli limitations, including the use of fusion partners that improve protein expression and solubility. New fusion technologies are emerging to complement the tradi...
P. brasiliensis virulence is affected by SconC, the negative regulator of inorg...
Menino, João F.; Saraiva, Margarida; Gomes, Jéssica Angélica Rezende; Sturme, Mark H. J.; Pedrosa, Jorge; Castro, António G.; Ludovico, PaulaConidia/mycelium-to-yeast transition of Paracoccidioidesbrasiliensis is a critical step for the establishment of paracoccidioidomycosis, a systemic mycosis endemic in Latin America. Thus, knowledge of the factors that mediate this transition is of major importance for the design of intervention strategies. So far, the only known pre-requisites for the accomplishment of the morphological transition are the tempe...
The Fh8 tag : a fusion partner for simple and cost-effective protein purificati...
Costa, Sofia Judite; Coelho, Eduardo; Franco, Lara Oliveira; Almeida, André; Castro, António G.; Domingues, LucíliaDownstream processing is still a major bottleneck in recombinant protein production representing most of its costs. Hence, there is a continuing demand of novel and cost-effective purification processes aiming at the recovery of pure and active target protein. In this work, a novel purification methodology is presented, using the Fh8 solubility enhancer tag as fusion handle. The binding properties of Fh8 tag to...
A novel adjuvant-free H fusion system for the production of recombinant immunog...
Costa, Sofia Judite; Silva, P.; Almeida, André; Conceição, A.; Domingues, Lucília; Castro, António G.The production of recombinant antigens in Escherichia coli and specific polyclonal antibodies for diagnosis and therapy is still a challenge for world-wide researchers. Several different strategies have been explored to improve both antigen and antibody production, all of them depending on a successful expression and immunogenicity of the antigen. Gene fusion technology attempted to address these challenges: fu...
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