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Sample richness and genetic diversity as drivers of chimera formation in nSSU m...

Fonseca, V. G.; Nichols, B.; Lallias, D.; Quince, C.; Carvalho, G .R.; Power, Deborah; Creer, S.

Eukaryotic diversity in environmental samples is often assessed via PCR-based amplification of nSSU genes. However, estimates of diversity derived from pyrosequencing environmental data sets are often inflated, mainly because of the formation of chimeric sequences during PCR amplification. Chimeras are hybrid products composed of distinct parental sequences that can lead to the misinterpretation of diversity es...

Data: 2012 | Origem: Sapientia - Universidade do Algarve

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Systematic and evolutionary insights derived from mtDNA COI barcode diversity i...

Matzen da Silva, J.; Creer, S.; Santos, A. dos; Costa, A. C.; Cunha, M. R.; Costa, Filipe O.; Carvalho, G. R.

Decapods are the most recognizable of all crustaceans and comprise a dominant group of benthic invertebrates of the continental shelf and slope, including many species of economic importance. Of the 17635 morphologically described Decapoda species, only 5.4% are represented by COI barcode region sequences. It therefore remains a challenge to compile regional databases that identify and analyse the extent and pa...

Data: 2011 | Origem: RepositóriUM - Universidade do Minho

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