Encontrados 16 documentos, a visualizar página 1 de 2
Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5
Santos, Ana I.; Carreira, Bruno P.; Nobre, Rui J.; Carvalho, Caetana M.; Araujo, Ines M.The involvement of nitric oxide (NO) and cyclic GMP (cGMP) in neurogenesis has been progressively unmasked over the last decade. Phosphodiesterase 5 (PDE5) specifically degrades cGMP and is highly abundant in the mammalian brain. Inhibition of cGMP hydrolysis by blocking PDE5 is a possible strategy to enhance the first step of neurogenesis, proliferation of neural stem cells (NSC). In this work, we have studied...
Evaluation of neurotoxic and neuroprotective pathways affected by antiepileptic...
Morte, Maria I.; Carreira, Bruno P.; Falcão, Maria J.; Ambrosio, António M.; Soares-da-Silva, Patrício; Araújo, Inês M.; Carvalho, Caetana M.In this study we evaluated the neurotoxicity of eslicarbazepine acetate (ESL), and of its in vivo metabolites eslicarbazepine (S-Lic) and R-licarbazepine (R-Lic), as compared to the structurally-related compounds carbamazepine (CBZ) and oxcarbazepine (OXC), in an in vitro model of cultured rat hippocampal neurons. The non-related antiepileptic drugs (AEDs) lamotrigine (LTG) and sodium valproate (VPA) were also ...
GDNF modulates HO-1 expression in substantia nigra postnatal cell cultures
Saavedra, Ana; Baltazar, Graça; Carvalho, Caetana M.; Duarte, Emília P.Heme oxygenase-1 (HO-1) has been strongly highlighted because of its induction in many cell types by toxic stimuli, including oxidative stress. The intense HO-1 immunostaining in the substantia nigra of Parkinson disease (PD) patients suggests its involvement in the pathogenesis of this neurodegenerative disease. In this work we investigated HO-1 expression in rat substantia nigra postnatal cell cultures under ...
Early calpain-mediated proteolysis following AMPA receptor activation compromis...
Araújo, Inês M.; Verdasca, Maria J.; Leal, Ermelindo C.; Bahr, Ben A.; Ambrósio, António F.; Carvalho, Arsélio P.; Carvalho, Caetana M.http://dx.doi.org/10.1111/j.1471-4159.2004.03043.x
Functional interaction between neuropeptide Y receptors and modulation of calci...
Silva, Ana P.; Carvalho, Arsélio P.; Carvalho, Caetana M.; Malva, João O.We investigated the functional interaction between neuropeptide Y (NPY) receptors using nerve terminals and cultured rat hippocampal neurons, and we evaluated the involvement of voltage-gated Ca2+ channels (VGCCs) in NPY receptors-induced inhibition of Ca2+ influx and glutamate release. The KCl-evoked release of glutamate from hippocampal synaptosomes was inhibited by 1 [mu]M NPY and this effect was insensitive...
Development of chick retina cells in culture: cobalt entry through AMPA recepto...
Cristóvão, Armando J.; Carvalho, Caetana M.The functionality of [alpha]-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors in chick embryo retina cells during development in vitro was studied by using Co2+ uptake, and these data were correlated with the expression of the AMPA receptor subunit GluR4. We found that at 5 h in vitro only a small number of cells took up Co2+ upon stimulation with 100 [mu]M kainate or other AMPA receptor agonists...
Activation of neuropeptide Y receptors is neuroprotective against excitotoxicit...
Silva, Ana P.; Pinheiro, Paulo S.; Carvalho, Arsélio P.; Carvalho, Caetana M.; Jakobsen, Birthe; Zimmer, Jens; Malva, João O.Glutamate and NPY have been implicated in hippocampal neuropathology in temporal lobe epilepsy. Thus, we investigated the involvement of NPY receptors in mediating neuroprotection against excitotoxic insults in organotypic cultures of rat hippocampal slices. Exposure of hippocampal slice cultures to 2 μM AMPA (α-amino-3-hydroxy-5-methyl-isoxazole-4- propionate) induced neuronal degeneration, monitored by propid...
Expression of AMPA/kainate receptors during development of chick embryo retina ...
Cristóvão, Armando J.; Oliveira, Catarina R.; Carvalho, Caetana M.The activity and the subunit expression of [alpha]-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA)/kainate ionotropic glutamate receptors were studied in retina cells developing in chick embryos and in retina cells cultured as retinospheroids, at the same stages of development. In the retinospheroids, the activity of the AMPA/kainate receptors was monitored by following the changes in the intracellular fr...
Mechanisms of Action of Carbamazepine and Its Derivatives, Oxcarbazepine, BIA 2...
Ambrósio, António F.; Soares-da-Silva, Patrício; Carvalho, Caetana M.; Carvalho, Arsélio P.Carbamazepine (CBZ) has been extensively used in the treatment of epilepsy, as well as in the treatment of neuropathic pain and affective disorders. However, the mechanisms of action of this drug are not completely elucidated and are still a matter of debate. Since CBZ is not very effective in some epileptic patients and may cause several adverse effects, several antiepileptic drugs have been developed by struc...
Inhibition of glutamate release by BIA 2-093 and BIA 2-024, two novel derivativ...
Ambrósio, António F.; Silva, Ana P.; Malva, João O.; Soares-da-Silva, Patricio; Carvalho, Arsélio P.; Carvalho, Caetana M.We investigated the mechanism(s) of action of two new putative antiepileptic drugs (AEDs), (S)-(-)-10-acetoxy-10,11-dihydro-5H-dibenz[b,f]azepine-5-carboxamide (BIA 2-093) and 10,11-dihydro-10-hydroxyimino-5H-dibenz[b,f]azepine-5-carboxamide (BIA 2-024), by comparing their effects on the release of endogenous glutamate in hippocampal synaptosomes, with those of carbamazepine (CBZ) and oxcarbazepine (OXC). The A...
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