Encontrados 6 documentos, a visualizar página 1 de 1
African swine fever virus p10 protein exhibits nuclear import capacity and accu...
Nunes-Correia, Isabel; Rodríguez, Javier M.; Eulálio, Ana; Carvalho, Ana L.; Citovsky, Vitaly; Simões, Sérgio; Faro, Carlos; Salas, María L.African swine fever virus (ASFV), a large enveloped DNA-containing virus, infects domestic and wild pigs, and multiplies in soft ticks, causing an economically relevant hemorrhagic disease. Evaluation of the nuclear import ability of ASFV p10 protein was the major purpose of the present work. Two approaches were used to determine if p10 protein is imported into the nucleus by an active process: a yeast-based nu...
Heterodimeric nitrate reductase (NapAB) from Cupriavidus necator H16: purificat...
Coelho, Catarina; J. Gonzaléz, Pablo; Trincão, José; Carvalho, Ana L.; Najmudin, Shabir; Moura, José J. G.; Hettman, Thomas; Dieckman, StephanActa Cryst. (2007). F63, 516–519 ; The periplasmic nitrate reductase from Cupriavidus necator (also known as Ralstonia eutropha) is a heterodimer that is able to reduce nitrate to nitrite. It comprises a 91 kDa catalytic subunit (NapA) and a 17 kDa subunit (NapB) that is involved in electron transfer. The larger subunit contains a molybdenum active site with a bis-molybdopterin guanine dinucleotide cofactor as...
Superoxide reductase from the syphilis spirochete Treponema pallidum
Moura, Isabel; Santos-Silva, Teresa; Trincão, José; Carvalho, Ana L.; Auchère, Françoise; Moura, José J. G.; Romão, Maria J.Superoxide reductase is a 14 kDa metalloprotein containing a catalytic nonhaem iron centre [Fe(His)4Cys]. It is involved in defence mechanisms against oxygen toxicity, scavenging superoxide radicals from the cell. The oxidized form of Treponema pallidum superoxide reductase was crystallized in the presence of polyethylene glycol and magnesium chloride. Two crystal forms were obtained depending on the oxidizing ...
Differential postreceptor signaling events triggered by excitotoxic stimulation...
Santos, Armanda E.; Carvalho, Ana L.; Lopes, Maria C.; Carvalho, Arsélio P.The aim of this work was to investigate whether excitotoxicity induced by overstimulation of different ionotropic glutamate receptors could trigger different intracellular signaling cascades. Cultured chick neuronal retina cells, essentially amacrine-like, were particularly sensitive to the toxicity induced by non-NMDA glutamate receptor agonists. One hour stimulation with 100 muM kainate induced a reduction of...
Glutamate in Life and Death of Retinal Amacrine Cells*
Duarte, Carlos B.; Ferreira, Ildete L.; Santos, Paulo F.; Carvalho, Ana L.; Agostinho, Paula M.; Carvalho, Arsélio P.1. Glutamate is the neurotransmitter released by bipolar cells at their synapses with amacrine cells. The amacrine cells express ionotropic (NMDA, AMPA and kainate) and metabotropic (mGluR1, mGluR2, mGluR4 and mGluR7) glutamate receptors and may take up glutamate from the synaptic cleft. ; http://www.sciencedirect.com/science/article/B6T71-3WDCGNH-6/1/585429baef39660288a51842ff1b4c33
Photoacoustic Measurements of Porphyrin Triplet-State Quantum Yields and Single...
Pineiro, Marta; Carvalho, Ana L.; Pereira, Mariette M.; Gonsalves, A. M. d'A. Rocha; Arnaut, Luís G.; Formosinho, Sebastião J.Photoacoustic calorimetry and luminescence techniques were used to measure singlet oxygen quantum yields (PhiDelta) of selected 5,10,15,20-tetrakis(aryl)porphyrins and of the novel chlorin shown. The use of validated photoacoustic references removes some inconsistencies existing in the literature. Excitation of halogenated porphyrins produces high yields of long-lived triplet states that efficiently transfer th...
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