Encontrados 4 documentos, a visualizar página 1 de 1
Novel method to quantify intracellular accumulation of polyphosphate in EBPR sy...
Mesquita, D. P.; Amaral, A. L.; Leal, Cristiano; Carvalheira, Mónica; Cunha, J. R.; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, E. C.A new method for intracellular storage polyphosphate (poly-P)identification and quantification in enhanced biological phosphorus removal (EBPR) systems is proposed based on image analysis. In EBPR systems, 4',6-diamidino-2-phenylindole (DAPI) is usually combined with fluororescent in situ hybridization (FISH) to evaluate the microbial community. The proposed technique is based on an image analysis procedure spe...
Biopolymer monitoring using quantitative image analysis techniques
Carvalheira, Mónica; Mesquita, D. P.; Leal, Cristiano; Oehmen, Adrian; Carvalho, Gilda; Cunha, J. R.; Amaral, A. L.; Ferreira, E. C.; Reis, Maria A. M.Polyhydroxyalkanoates (PHAs) are intracellular biopolymers with many applications, particularly as substitutes of polypropylene and polyethylene, due to their thermoplastic properties and biocompatible nature. Furthermore, glycogen is a polysaccharide of glucose with high importance in the metabolism of microbial communities and polyphosphate is a microbial storage compound that should be recovered in order to ...
Intracellular poly-P assessment by DAPI staining and image analysis
Amaral, A. L.; Mesquita, D. P.; Leal, C.; Carvalheira, Mónica; Oehmen, Adrian; Reis, Maria A. M.; Ferreira, E. C.In wastewater treatment, enhanced biological phosphorus removal (EBPR) is considered a well-established process to remove phosphate (P). EBPR is based on the activity of polyphosphate-accumulating organisms (PAOs) able to take up and store large amounts of P as intracellular (poly-P) granules. However, monitoring poly-P in mixed cultures is usually performed by a laborious and time consuming off-line chemical a...
Multimédia interativo como suporte à comunicação em saúde
Carvalheira, Mónica AntunesA presente investigação visa compreender como é possível através de uma aplicação multimédia influenciar a comunicação em saúde. Neste sentido, pretende-se desenvolver uma proposta de estratégia de comunicação para crianças com diabetes tipo 1. Com o objetivo de comprovar as hipóteses traçadas foram entrevistadas crianças com idades compreendidas entre os 6 e os 10 anos portadoras de diabetes tipo 1 acompanhada...
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