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Lysis buffer properties : influence on S. epidermidis biofilm proteome analysis
Carvalhais, V.; Cerca, Nuno; Vitorino, RuiBesides being part of human commensal flora, S. epidermidis has the ability to colonize and form biofilms in artificial implants. Due to the particular characteristics of biofilms, conventional methods used to disrupt and lyses biofilms from Gram positive bacteria may include association between mechanical, enzymatic and chemical methods. Nevertheless, proteomic characterization is highly dependent of the extra...
Global transcriptomic analysis of dormancy within Staphylococcus epidermidis bi...
Carvalhais, V.; França, Ângela; Vitorino, Rui; Pier, Gerald B.; Vilanova, Manuel; Cerca, Nuno
Whole transcriptome analysis of Staphylococcus epidermidis biofilms upon contac...
França, Ângela; Carvalhais, V.; Maira-Litrán, Tomas; Vilanova, Manuel; Cerca, Nuno; Pier, Gerald B.Staphylococcus epidermidis is commonly associated with foreign body infections due to its ability to form biofilms on the surface of indwelling medical devices. Understanding how the biofilms form on the top of devices and interact with the host, particularly, with human blood components, is of great interest. Here we assessed the whole transcriptome of S. epidermidis biofilms upon contact with human blood. Est...
A first new look into the interaction of Staphylococcus epidermidis biofilm-rel...
França, Ângela; Pérez-Cabezas, B.; Carvalhais, V.; Freitas, Ana I.; Pier, G.; Vilanova, Manuel; Cerca, NunoThe widespread application of indwelling medical devices in the clinical setting, together with the remarkable ability of the commensal Staphylococcus epidermidis to adhere to these surfaces and form biofilms, has given to this bacterium the recognition of being a leading causative agent of nosocomial infections. Biofilms lifecycle is currently divided into 4 main steps: initial adhesion, accumulation, maturati...
Medium supplementation with magnesium prevented the induction of dormancy in bi...
Pérez-Cabezas, B.; Oliveira, C.; Carvalhais, V.; Freitas, Ana I.; Oliveira, F.; Cerca, Nuno; Vilanova, ManuelStaphylococcus epidermidis is a commensal bacterium that colonizes the skin and mucous membranes, being the most prevalent staphylococcal species found in humans. However, S. epidermidis has the ability of colonize indwelling medical devices establishing biofilms, which makes this bacteria a common cause of bacteraemia particularly in immunocompromised individuals and neonates [1][2]. The presence of high amoun...
Staphylococcus epidermidis biofilm lifecycle and its virulence: from plantkonic...
França, Ângela; Freitas, Ana I.; Carvalhais, V.; Pérez-Cabezas, B.; Correia, A.; Cywes-Bentley, C.; Maira-Litrán, Tomas; Vilanova, M.; Pier, Gerald B.
Staphylococcus epidermidis biofilm dispersal cells : an intermediary phenotype?
França, Ângela; Freitas, Ana I.; Carvalhais, V.; Cywes-Bentley, C.; Maira-Litrán, Tomas; Vilanova, M.; Cerca, Nuno
Effect of S. epidermidis biofilm matrix in RNA quality gene expression determin...
Carvalhais, V.; Delgado-Rastrollo, M.; Cerca, Nuno
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