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Overproduction, crystallization and preliminary X-ray characterization of Abn2,...
Sá-Nogueira, Isabel de; Sanctis, Daniele de; Bento, Isabel; Inácio, José Manuel; Custódio, Sónia; Carrondo, Maria ArméniaActa Crystallographica F64 (2008) 636-638 ; Two Bacillus subtilis extracellular endo-1,5-alpha-L-arabinanases, AbnA and Abn2, belonging to glycoside hydrolase family 43 have been identified. The recently characterized Abn2 protein hydrolyzes arabinan and has low identity to other reported 1,5-alpha-L-arabinanases. Abn2 and its selenomethionine (SeMet) derivative have been purified and crystallized. Crystals ap...
The Glycosylation of the Aspartic Proteinases from Barley (Hordeum Vulgare L.) ...
Costa, Júlia; Ashford, David A.; Nimtz, Manfred; Bento, Isabel; Frazão, Carlos; Esteves, Cristina L.; Faro, Carlos J.; Kervinen, Jukka; Pires, EuclidesPlant aspartic proteinases characterised at the molecular level contain one or more consensus N-glycosylation sites [Runeberg-Roos, P., Törmäkangas, K. & Östman, A. (1991) Eur. J. Biochem. 202, 102120131027; Asakura, T., Watanabe, H., Abe, K. & Arai, S. (1995) Eur. J. Biochem. 232, 77201383; Veríssimo, P., Faro, C., Moir, A. J. G., Lin, Y., Tang, J. & Pires, E. (1996) Eur. J. Biochem. 235, 76220137681. We found...
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