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Structure of ß-cinnamomin, a protein toxic to some plant species
Rodrigues, Maria Luisa; Archer, Margarida; Martel, Paulo; Jacquet, Alain; Cravador, A.; Carrondo, Maria A.Phytophthora and Pythium species are among the most aggressive plant pathogens, as they invade many economically important crops and forest trees. They secrete large amounts of 10 kDa proteins called elicitins that can act as elicitors of plant defence mechanisms. These proteins may also induce a hypersensitive response (HR) including plant cell necrosis, with different levels of toxicity depending on their pI....
Crystallisation and preliminary X-ray diffraction analysis of alpha-cinnamomin,...
Archer, Margarida; Rodrigues, Maria Luisa; Aurélio, M.; Biemans, R.; Cravador, A.; Carrondo, Maria A.Cinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapourdiffusion method using either ammonium sulfate or polyethyleneglycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are i...
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