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DNA molecular solvation in neat ionic liquids

Decolourisation of a synthetic textile effluent using a bacterial consortium

In the present study we examined the performance of a thermoalkalophilic bacterial consortium, where the predominant strain was Bacillus sp. SF, in the degradation of Reactive Black 5 (RB5). We used a reactor working in continuous mode and investigated the effects of pH, hydraulic retention time (HRT) and several added salts on colour and chemical oxygen demand (COD) reductions. For the chosen operational condi...

Azo reductase activity of intact saccharomyces cerevisiae cells Is dependent on...

Unspecific bacterial reduction of azo dyes is a process widely studied in correlation with the biological treatment of colored wastewaters, but the enzyme system associated with this bacterial capability has never been positively identified. Several ascomycete yeast strains display similar decolorizing behaviors. The yeastmediated process requires an alternative carbon and energy source and is independent of pr...

Azo reductase activity of intact Saccharomyces cerevisiae cells is dependent on...

Unspecific bacterial reduction of azo dyes is a process widely studied in correlation with the biological treatment of coloured wastewaters, but the enzyme system associated with this bacterial capability has never been positively identified. Several ascomycete yeast strains display similar decolourising behaviours. The yeast-mediated process requires an alternative carbon and energy source and is independent o...

Role of the component Fre1p of the plasma membrane ferric reductase on the azo ...

In Press. Aceite em 2005 para publicação na revista Applied and Environmental Microbiology. ; Unspecific bacterial reduction of azo dyes is a widely studied process in correlation with the biological treatment of coloured waste waters but the enzyme system associated to this bacterial capability has never been positively identified. Several ascomycete yeast strains display similar decolourising activities. The...

The azoreductase of yeast cells : a new feature of an old enzyme

Apresentação efectuada nas Jornadas de Biologia de Leveduras “Professor Nicolau van Uden”, 12, Universidade de Aveiro, Portugal, 13-15 Maio 2004. ; In an attempt to develop a biological treatment for colour removal we isolated several ascomycete yeast strains from contaminated soil based on its capacity to decolourize soluble azo dyes. We studied the process in batch cultures and have demonstrated that the col...

Is Saccharomyces cerevisiae azoreductase the plasma membrane ferric reductase?

Poster apresentado na International Conference on Plasma Membrane Redox Systems and their Role in Biological Stress and Disease, 7, Asilomar, Califórnia, Estados Unidos da América, 14-18 Abril 2004. ; Unspecific bacterial reduction of azo dyes is a widely studied process in correlation with the biological treatment of coloured waste waters but the enzyme system associated to this bacterial capability has never...

Isolation of an acetyl-CoA synthetase gene (ZbACS2) from Zygosaccharomyces bailii

A gene homologous to Saccharomyces cerevisiae ACS genes, coding for acetyl-CoA synthetase, has been cloned from the yeast Zygosaccharomyces bailii ISA 1307, by using reverse genetic approaches. A probe obtained by PCR amplification from Z. bailii DNA, using primers derived from two conserved regions of yeast ACS proteins, RIGAIHSVVF (ScAcs1p; 210–219) and RVDDVVNVSG (ScAcs1p; 574–583), was used for screening a ...

Characterization of azo reduction activity in a novel ascomycete yeast strain

Several model azo dyes are reductively cleaved by growing cultures of an ascomycete yeast species, Issatchenkia occidentalis. In liquid media containing 0.2 mM dye and 2% glucose in a mineral salts base, more than 80% of the dyes are removed in 15 h, essentially under microaerophilic conditions. Under anoxic conditions, decolorization does not occur, even in the presence of pregrown cells. Kinetic assays of azo...

Predicting dye biodegradation from redox potentials

Two biological approaches for decolorization of azo sulfonated dyes have been compared: reductive decolorization with the ascomycete yeast Issatchenkia occidentalis and nzymatic oxidative decolorization with Trametes villosa laccase alone or in the presence of the mediator 1-hydroxybenzotriazole. The redox potential difference between the biological cofactor involved in the reductive activity of growing cells ...