Encontrados 10 documentos, a visualizar página 1 de 1
A degenerate pair of primers for simultaneous detection of four alphaand betane...
Varanda, Carla; Cardoso, Joana M.S.; Oliveira, Mônica; Oliveira, Solange; Clara, Maria Ivone; Félix, Maria RosárioThe high infection levels due to Olive latent virus 1 (OLV-1), Olive mild mosaic virus (OMMV) (alphanecrovirus) and Tobacco necrosis virus D (TNV-D) (betanecrovirus) in Portuguese olive orchards prompted us to develop a rapid PCR-based assay for the simultaneous detection of these viruses aimed at the sanitary selection and marketing of plant material in compliance with European Union regulations. A pair of deg...
First characterization of infectious cDNA clones of Olive mild mosaic virus
Cardoso, Joana M.S.; Félix, M. Rosário; Clara, M. Ivone; Oliveira, SolangeFull-length cDNA clones of an Olive mild mosaic virus (OMMV) isolate were constructed in order to find infectious cDNA clones. The sequencing of three individual full-length clones revealed some differences between them. In vitro transcription of these clones was performed and the effect of spontaneous mutations in the biological behaviour of the in vitro transcripts was evaluated by symptomatology, RNA accumul...
Multiplex RT-PCR for detection and identification of three necroviruses that in...
Varanda, Carla; Cardoso, Joana M.S.; Félix, M. Rosário; Oliveira, Solange; Clara, M.IvoneAn optimized multiplex RT-PCR assay was developed to discriminate three necrovirus (Olive latent virus 1 (OLV-1), Tobacco necrosis virus D (TNV-D) and Olive mild mosaic virus (OMMV)) that infect olive trees. An olive orchard consisting of 54 trees of cv. "Galega vulgar" in the south of Portugal was surveyed. dsRNA fraction was used as template and revealed the 3 viruses, singly or in multiple infections, presen...
Biological and Molecular Characterization of Olive latent virus 1
Félix, M. Rosário; Cardoso, Joana M.S.; Oliveira, Solange; Clara, M. Ivone E.Olive latent virus 1 (OLV-1) belongs to the Necrovirus genus, Tombusviridae family and is pathogenic to olive, citrus and tulip plants. It is easily mechanically transmissible to indicator plants causing necrotic lesions and can be transmitted through the soil into the plant roots in the absence of biological vectors. Infected cells contain virus aggregates, inclusions made up of excess of viral coded peptides ...
Biological and Molecular Characterization of Olive latent virus 1
Félix, M. Rosário; Cardoso, Joana M.S.; Oliveira, Solange; Clara, M. Ivone E.Olive latent virus 1 (OLV-1) belongs to the Necrovirus genus, Tombusviridae family and is pathogenic to olive, citrus and tulip plants. It is easily mechanically transmissible to indicator plants causing necrotic lesions and can be transmitted through the soil into the plant roots in the absence of biological vectors. Infected cells contain virus aggregates, inclusions made up of excess of viral coded peptides ...
The complete genome sequence of a new necrovirus isolated from Olea europaea L.
Cardoso, Joana M. S.; Félix, M. Rosário; Clara, M. Ivone; Oliveira, SolangeThe complete nucleotide sequence of a virus isolated from Olea europaea L. (GP isolate), previously identified as an isolate of Tobacco necrosis virus D (TNV-D) based on its coat protein sequence, was determined. The viral RNA genome consists of 3683 nucleotides and contains five open reading frames. The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity with that of an isolate of Olive laten...
Viral properties, primary structure and phylogenetic analysis of the coat prote...
Félix, M. Rosário; Cardoso, Joana M. S.; Oliveira, Solange; Clara, M. IvoneAn Olive latent virus 1 isolate designated GM6, obtained from a Portuguese olive tree, was characterized and the coat protein gene sequenced and analysed. The purified virus particles showed to be isometric with ca.30 nm in diameter and contained a single-stranded RNA species with ca. 3.7 kb. The dsRNA profile obtained from infected tissues showed three major species with ca. 3.7, 1.5 and 1.3 kbp. SDS-PAGE anal...
The complete genome sequence of a new necrovirus isolated from Olea europaea L.
Cardoso, Joana M.S.; Félix, M.Rosário; Clara, M. Ivone E.; Oliveira, SolangeAbstract: The complete nucleotide sequence of a virus isolated from Olea europaea L. (GP isolate), previously identified as an isolate of Tobacco necrosis virus D (TNV-D) based on its coat protein sequence, was determined. The viral RNA genome consists of 3683 nucleotides and contains five open reading frames. The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity with that of an isolate of O...
Complete nucleotide sequence of an Olive latent virus 1 isolate from olive trees
Félix, M. Rosário; Cardoso, Joana M.S.; Varanda, Carla M.R.; Oliveira, Solange; Clara, M. Ivone E.Olive latent virus 1 (OLV-1) is a necrovirus belonging to the familyTombusviridae. It is a small icosahedral plant virus, which encapsidates a single stranded positivesense RNA. This virus was first isolated from symptomless olive trees in Italy [7] and afterwards in Jordan and Portugal [10, 4]. OLV-1 was also isolated from symptomatic hosts, such as citrus trees in Turkey [11] and tulips in Japan [9]. Up to no...
A Tobacco necrosis virus D isolate from Olea europaea L.: viral characterizatio...
Cardoso, Joana M. S.; Félix, M. Rosário; Oliveira, Solange; Clara, M. IvoneA virus isolated from Olea europaea L. grown in Portugal, was identified as a member of the species Tobacco necrosis virus D (TNV-D, genus Necrovirus, family Tombusviridae), based on the molecular and serological properties of the purified virus particles. The genomic region encoding the coat protein (CP) of this isolate (named GP isolate) was amplified by RT-PCR and the cDNA was cloned and sequenced. The CP ge...
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