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Genetic Transformation of V. vinifera - Two Alternative Systems under Evaluation

‘Trincadeira’ and ‘Aragonez’ are two of the most important Vitis vinifera L. cultivars used for red wine production in Portugal. Aiming to obtain a reproducible system for plant genetic transformation, the techniques of particle bombardment and transformation mediated by Agrobacterium tumefaciens were tested. In both systems putative transformed embryos were selected by continued proliferation on an in vitro me...

Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europa...

Alternative oxidase (AOX) has been proposed as a functional marker candidate in a number of events involving cell differentiation, including rooting efficiency in semi-hardwood shoot cuttings of olive (Olea europaea L.). To ascertain the general importance of AOX in olive rooting, the auxin-induced rooting process was studied in an in vitro system for microshoot propagation. Inhibition of AOX by salicylhydroxa...

Expression studies of AOX during adventitious root induction in Olea europaea L.

Recently, identification of four alternative oxidase (AOX) gene sequences from olive (Olea europaea L.) has been reported from our group. Three genes could be classified as members of the sub-family AOX1 and one as belonging to the sub-family of AOX2. AOX has been proposed as functional marker candidate for easy-to-root olive genotypes. Validation of AOX as an appropriate marker candidate needs to highlight a c...

Adesão e sucesso terapêutico

Use of morphometric parameters for tracking ovule and microspore evolution in g...

Somatic embryogenesis induction from in vitro cultured stamens and carpels is highly dependent on explants’ inoculation at specific developmental stages. To establish good correlations between measurable morphometric parameters of flowers or flower buds and developmental stages of micro- and macrosporogenesis, this procedure is the easiest way to simplify the in vitro culture procedures. These correlations were...

Daucus carota L. – An old model for cell reprogramming gains new importance thr...

The paper highlights Daucus carota L. as an ideal model to complement plant stress research on Arabidopsis thaliana L. Recently, alternative oxidase (AOX) is discussed as functional marker candidate for cell reprogramming upon stress. Carrot is the most studied species for cell reprogramming and our current research reveals that it is the only one that has expanded both AOX sub-family genes. We point to recentl...

Simplifying procedures to increase competitiveness at in vitro propagation of t...

With the aim to reduce fees of a micropropagation protocol for the Portuguese olive cultivar ‘Galega Vulgar’, expensive Difco Bacto-Agar® was replaced by commercial agar-agar, and the analysed data revealed no significant differences between the two agars, for both the explants multiplication rate and the rooting capacity. The replacement of the OM vitamin complex by Tonosol®, a commercial vitamin formulation, ...

Trends in adult stature of peoples who inhabited the modern Portuguese territor...

This study documents long-term changes in stature from the Mesolithic to the late 20th century in the territory of modern Portugal. Data utilised originated from published sources and from a sample of the Lisbon identified skeletal collection, where long bone lengths were collected. Mean long bone lengths were obtained from 20 population samples and compiled into nine periods. Pooled long bone lengths for each ...

Tiroidite auto-imune, experiência de uma consulta

Introdução: A doença auto-imune da tiróide é a principal causa de Hipotiroidismo adquirido na criança e adolescente, a sua incidência em idade escolar é de 1.3%1. Objectivo: Caracterizar os doentes com Tiroidite Auto-Imune da consulta de Endocrinologia Pediátrica do Hospital Geral de Santo António em seguimento no ano de 2006. Metodologia: Foi efectuado um estudo retrospectivo com revisão dos processos clínicos...

Isolation of alternative oxidase (AOX) genes of Olea europaea L.

Alternative oxidase (AOX) is recently suggested to be a potential candidate as functional marker for efficient cell reprogramming under stress (Arnholdt-Schmitt et al., 2006a). The presented work is part of a Marie Curie Chair project, that was established to investigate the potential role of the multigene AOX to assist breeding on efficient rooting of olive shoot cuttings (Arnholdt-Schmitt et al. 2006b). Plant...