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Carrot alternative oxidase gene AOX2a demonstrates allelic and genotypic polymo...
Cardoso, Hélia Guerra; Campos, Maria Doroteia; Costa, Ana Rita; Campos, Maria Catarina; Nothnagel, Thomas; Arnholdt-Schmitt, BirgitSingle nucleotide polymorphisms (SNPs) and insertion–deletions (InDels) are becoming important genetic markers for major crop species. In this study, we focus on variations at genomic level of the Daucus carota L. AOX2a gene. The use of gene- specific primers designed in exon regions on the boundaries of introns permitted to recognize intron length polymorphism (ILP) in intron 3 AOX2a by simple polymerase chain...
The alternative oxidase family of Vitis vinifera reveals an attractive model to...
Costa, José Hélio; Fernandes de Melo, Dirce; Gouveia, Zélia; Cardoso, Hélia Guerra; Peixe, Augusto; Arnholdt-Schmitt, BirgitGenomic design' refers to the structural organization of gene sequences. Recently, the role of intron sequences for gene regulation is being better understood. Further, introns possess high rates of polymorphism that are considered as the major source for speciation. In molecular breeding, the length of gene-specific introns is recognized as a tool to discriminate genotypes with diverse traits of agronomic inte...
Intron polymorphism pattern in AOX1b of wild St John's wort (Hypericum perforat...
Ferreira, Alexandre Oliveira; Cardoso, Hélia Guerra; Santos Macedo, Elisete; Breviario, Diego; Arnholdt-Schmitt, BirgitThe present paper deals with the analysis of natural polymorphism in a selected alternative oxidase (AOX) gene of the medicinal plant, St John's wort. Four partial AOX gene sequences were isolated from the genomic DNA of a wild plant of Hypericum perforatum L. Three genes belong to the subfamily AOX1 (HpAOX1a, b and c) and one to the subfamily AOX2 (HpAOX2). The partial sequence of HpAOX1b showed polymerase cha...
The gymnosperm Pinus pinea contains both AOX gene subfamilies, AOX1and AOX2
Frederico, António Miguel; Zavattieri, Maria Amely; Campos, Maria Doroteia; Cardoso, Hélia Guerra; McDonald, Allison E.; Arnholdt-Schmitt, BirgitThe gymnosperm Pinus pinea L. (stone pine) is a typical Mediterranean pine used for nuts and timber production,and as an ornamental around the world. Pine genomes are large in comparison to other species. The hypothesis that retrotransposons, such as gymny, made a large contribution to this alteration in genome size was recently confirmed. However, P. pinea is unique in other various aspects. P. pinea demonstra...
Differential expression and co-regulation of carrot AOX genes (Daucus carota)
Campos, Maria Doroteia; Cardoso, Hélia Guerra; Linke, Bettina; Costa, José Hélio; Fernandes de Melo, Dirce; Justo, Lígia; Frederico, António MiguelAlternative oxidase (AOX) is a mitochondrial protein encoded by the nuclear genome. In higher plants AOX genes form a small multigene family mostly consisting of the two subfamilies AOX1 and AOX2. Daucus carota L. is characterized by a unique extension pattern of AOX genes. Different from other plant species studied so far it contains two genes in both subfamilies. Therefore, carrot was recently highlighted as ...
Alternative oxidase involvement in Daucus carota somatic embryogenesis
Frederico, António Miguel; Campos, Maria Doroteia; Cardoso, Hélia Guerra; Imani, Jafargholi; Arnholdt-Schmitt, BirgitPlant alternative oxidase (AOX) is a mitochondrial inner membrane enzyme involved in alternative respiration.The critical importance of the enzyme during acclimation upon stress of plant cells is not fully understood and is still an issue of intensive research and discussion. Recently, a role of AOX was suggested for the ability of plant cells to change easily its fate upon stress. In order to get new insights ...
AOX – A potential marker for efficient rooting of olive shoot cuttings.
Arnholdt-Schmitt, Birgit; Santos Macedo, Elisete; Cardoso, Hélia Guerra; Peixe, Augusto; Cordeiro, António M.Alternative oxidase (AOX) is recently suggested to be a potential candidate as functional marker for efficient cell reprogramming under stress (Arnholdt-Schmitt et al., 2006a). The presented work is part of a Marie Curie Chair project, that was established to investigate the potential role of the multigene AOX to assist breeding on efficient rooting of olive shoot cuttings (Arnholdt-Schmitt et al. 2006b). Plant...
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