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A new approach to bacterial colony morphotyping by matrix-assisted laser desorp...
Sousa, Ana Margarida; Nunes-Miranda, J. D.; Reboiro-Jato, M.; Fdez-Riverola, Florentino; Lourenço, Anália; Pereira, M. O.; Capelo, José LuisMatrix assisted laser desorption ionization time of flight mass spectrometry has been explored as a tool to bacterial colony morphotyping.To this end,four colony morphotypes of Pseudomonas aeruginosa and four of Staphylococcus aureus were analysed using intact bacteria.Results suggest that mass spectrometry of intact bacteria could,in some extent,be used to complement the classical morphological classification ...
Synthesis, characterization, photophysical studies and interaction with DNA of ...
Pedras, Bruno; Batista, Rosa M. F.; Tormo, Laura; Costa, Susana P. G.; Raposo, M. Manuela M.; Orellana, Guillermo; Capelo, José Luis; Lodeiro, CarlosA new family of Ru(II) polypyridyl complexes (C1 to C6) containing furyl- or thienyl-imidazo-phenanthroline ligands (4-6) were synthesized using microwave irradiation and characterized by elemental analysis, 1H-NMR, UV-vis absorption and fluorescence spectroscopy, FAB, EI-MS and MALDI-TOF-MS spectrometry. On the other hand, the novel furyl- or thienyl-imidazo-phenanthroline derivatives (5-6) were synthesized th...
Timely and effectively profile bacteria in cystic fibrosis lungs
Sousa, A. M.; Lourenço, Anália; Fdez-Riverola, Florentino; Diniz, Júlio; Capelo, José Luis; Pereira, M. O.Bacterial lung infections are typical of cystic fibrosis (CF) disease due to accumulation of airway mucus. Despite the use of aggressive antibiotic therapy, the mortality rate of CF patients is still high. Unsuccessful bacterial eradication is often due to several evolutional strategies adopted by bacteria to achieve anaerobic or microaerophilic adaptation and antibiotic resistance, such as biofilm formation an...
Synthesis, characterization and spectroscopic studies of two new schiff-base bi...
Pedras, Bruno; Fernandes, Luz; Oliveira, Elisabete; Rodríguez, Laura; Raposo, M. Manuela M.; Capelo, José Luis; Lodeiro, CarlosNickel(II); Palladium(II); Mercury(II); Sodium(I); ; Crown ether, Schiff base, Luminescence ; Two new ligands provided with a 15-crown-5 as receptor unit and bithiophen unit as emissive probe have been synthesized and characterized in order to evaluate the coordination capabilities and their sensor effect. Ligand L1 presents an aromatic crown ether moiety directed linked to the imine-2,2´-bithiophene π–conjuga...
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