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A 32-KDa kilodalton hydrolase plays an important role in Paracoccidioides brasi...
Hernández, Orville; Almeida, A. J.; Gonzalez, Angel; Garcia, Ana Maria; Tamayo, Diana; Cano, L. E.; Restrepo, A.; McEwen, J. G.One of the most crucial events during infection with the dimorphic fungus Paracoccidioides brasiliensis is adhesion to pulmonary epithelial cells, a pivotal step in the establishment of disease. In this study, we have evaluated the relevance of a 32-kDa protein, a putative adhesion member of the haloacid dehalogenase (HAD) superfamily of hydrolases, in the virulence of this fungus. Protein sequence analyses hav...
New insights into the cell cycle profile of Paracoccidioides brasiliensis
Almeida, A. J.; Martins, M.; Carmona, J. A.; Cano, L. E.; Restrepo, A.; Leão, Cecília; Rodrigues, Fernando José dos SantosThe present work focuses on the analysis of cell cycle progression of Paracoccidioides brasiliensis yeast cells under diVerent environmental conditions. We optimized a Xow cytometric technique for cell cycle proWle analysis based on high resolution measurements of nuclear DNA. Exponentially growing cells in poor-deWned or rich-complex nutritional environments showed an increased percentage of daughter cells in ...
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