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In vitro propagation of the wild carrot Daucus carota L. subsp. halophilus (Bro...
Tavares, Ana Cristina; Salgueiro, Lígia R.; Canhoto, Jorge M.Daucus carota subsp. halophilus, is a wild crop relative of domestic carrot. It is an aromatic plant widely used in folk medicine due to recognized therapeutic properties of its essential oils. Experiments were carried out to evaluate the potential of in vitro propagation techniques to the conservation of this endemic and endangered taxon. The results showed that shoot tips of in vitro germinated seeds were abl...
Essential oils from Distichoselinum tenuifolium: Chemical composition, cytotoxi...
Tavares, Ana Cristina; Gonçalves, Maria José; Cruz, Maria Teresa; Cavaleiro, Carlos; Lopes, Maria Celeste; Canhoto, Jorge; Ribeiro Salgueiro, LigiaEthnopharmacological relevance: Several aromatic plants and their essential oils are known to possess antimicrobial and anti-inflammatory properties. Distichoselinum tenuifolium (Lag.) Garcia Martin & Silvestre, an Iberian endemism, is traditionally used in the treatment of contact dermatitis and skin infections. However, the cellular mechanisms through which this plant exerts their beneficial effects are not k...
Essential oil of Daucus carota subsp. halophilus: Composition, antifungal activ...
Tavares, Ana Cristina; Gonçalves, Maria José; Cavaleiro, Carlos; Cruz, Maria Teresa; Lopes, Maria Celeste; Canhoto, Jorge; Ribeiro Salgueiro, LigiaEthnopharmacological relevance: Essential oils are known to possess antimicrobial activity against a wide spectrum of bacteria and fungi. Daucus carota L. is used since olden times in traditional medicine, due to recognized therapeutic properties, namely the antimicrobial activity of their essential oils. Aim of the study: In the present study the composition and the antifungal activity of the oils of Daucus ca...
Testing the Use of the Water Milfoil ( Myriophyllum spicatum L.) in Laboratory ...
Sánchez, David; Graça, Manuel; Canhoto, JorgeAbstract Tests aiming to determine the toxic properties of compounds discharged into aquatic systems have relied more on fish or invertebrates than on primary producers and among a number of producers; algae are the most popular test organisms. Macrophytes are important ecological elements in freshwaters and are therefore potentially key organisms for use in toxicity testing of compounds suspected of acting in...
Somatic embryogenesis and plant regeneration in carob ( Ceratonia siliqua L.)
Canhoto, Jorge; Rama, Sandra; Cruz, GilSummary Somatic embryos of carob (Ceratonia siliqua L.) were induced from cotyledonary segments excised from immature seeds when cultured on Murashige and Skoog media supplemented with several combinations of 6-benzylaminopurine (BA) and indole-3-butyric acid (IBA). The best frequencies of induction (33.8%) were obtained when 4.4 µM BA and 0.5 µM IBA were used. Shoots were also sporadically formed in the same ...
Micropropagation of Eucalyptus nitens maiden (Shining gum)
Gomes, Filomena; Canhoto, JorgeSummary Eucalyptus nitens Maiden (shining gum) is a frost-tolerant species of Eucalyptus that can be used as an alternative species to Eucalyptus globulus in some regions of Portugal where winter temperatures are too low. Seedlings and 1-yr-old shoot tips and nodes were used for micropropagation of E. nitens. The best multiplication rate (2.25) was obtained when seedling shoots (<15 mm) were cultured on a ...
Somatic embryogenesis and plant regeneration in myrtle (Myrtaceae)
Canhoto, Jorge; Lopes, Maria; Cruz, GilAbstract Somatic embryos of myrtle (Myrtus communis L.) were induced from mature zygotic embryos cultured in MS medium supplemented with several concentrations of 2,4-D (2.26 µM – 18.98 µM) or Picloram (2.07 µM – 16.5 µM) combined with 0.087 M or 0.23 M sucrose. For all the concentrations of 2,4-D or Picloram tested, 0.087 M sucrose proved to be more effective than 0.23 M. The best frequencies of induction wer...
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