Encontrados 21 documentos, a visualizar página 1 de 3
Contribution of Yap 1 towards S. cervisiae adaptation to arsenic mediated oxida...
Menezes, Regina A.; Amaral, Catarina; Batista-Nascimento, Liliana; Santos, Cláudia; Ferreira, Ricardo Boavida; Devaux, Fréderic; Eleutherio, Elis C.A.Post-Print ; In the budding yeast Saccharomyces cerevisiae arsenic detoxification involves the activation Yap8, a member of the Yap family of transcription factors, which in turn regulates ACR2 and ACR3, encoding an arsenate reductase and a plasma membrane arsenite efflux-protein, respectively. In addition, Yap1 is involved in the arsenic adaptation process through regulating the expression of the vacuolar-pum...
Characterization and expression analysis of the aspartic protease gene family o...
Pimentel, Catarina; Straeten, Dominique Van Der; Pires, Euclides; Faro, Carlos; Rodrigues-Pousada, ClaudinaCardosin A and cardosin B are two aspartic proteases mainly found in the pistils of cardoon Cynara cardunculus L., whose flowers are traditionally used in several Mediterranean countries in the manufacture of ewe's cheese. We have been characterizing cardosins at the biochemical, structural and molecular levels. In this study, we show that the cardoon aspartic proteases are encoded by a multigene family. The ge...
Molecular determinants for FMN-binding in Desulfovibrio gigas flavoredoxin
Broco, Manuela; Soares, Claudio M.; Oliveira, Solange; Mayhew, Stephen G; Rodrigues-Pousada, ClaudinaAbstract: Flavoredoxin participates in Desulfovibrio gigas thiosulfate reduction pathway. Its 3-dimensional model was generated allowing the oxidized riboflavin-5'-phosphate (FMN) site to be predicted. Residues likely to be involved in FMN-binding were identified (N29, W35, T56, K92, H131 and F164) and mutated to alanine. Fluorescence titration with apoprotein showed that FMN is strongly bound in the wild-type ...
A chemotaxis operon in the bacterium Desulfovibrio gigas is induced under sever...
Félix, Rute; Rodrigues, Rute; Machado, Patrícia; Oliveira, Solange; Rodrigues-Pousada, ClaudinaAbstract: The chemosensory system of bacteria controls their motility and behaviour in different environments. In the present study, we report the identification of the first chemotaxis operon in Desulfovibrio gigas. Amino acid sequence analysis revealed seven coding regions for polypeptides with a high similarity to chemotaxis proteins from other organisms. D. gigas chemotaxis operon has a similar genetic orga...
Characterization and expression analysis of the cytochrome bd oxidase operon fr...
Machado, Patrícia; Félix, Rute; Rodrigues, Rute; Oliveira, Solange; Rodrigues-Pousada, ClaudinaAbstract: Although classified as anaerobic, Desulfovibrio gigas contains a functional canonical membrane respiratory chain, including a cytochrome bd quinol oxidase as its terminal element. In the present study, we report the identification of the operon cydAB encoding the two subunits of cytochrome bd from this bacterium. Two hypothetical promoter regions and sequences resembling transcriptional regulators-bin...
Desulfovibrio gigas flavodiiron protein affords protection against nitrosative ...
Rodrigues, Rute; Vicente, João B.; Félix, Rute; Oliveira, Solange; Teixeira, Miguel; Rodrigues-Pousada, ClaudinaAbstract: Desulfovibrio gigas flavodiiron protein (FDP), rubredoxin: oxygen oxidoreductase (ROO), was proposed to be the terminal oxidase of a soluble electron transfer chain coupling NADH oxidation to oxygen reduction. However, several members from the FDP family, to which ROO belongs, revealed nitric oxide (NO) reductase activity. Therefore, the protection afforded by ROO against the cytotoxic effects of NO w...
Characterisation of the 11Kb DNA region adjacent to the gene encoding Desulfovi...
Broco, Manuela; Marques, Ana; Oliveira, Solange; Rodrigues-Pousada, ClaudinaAbstract: Flavoredoxin is an FMN binding protein that functions as an electron carrier in the sulphate metabolism of Desulfovibrio gigas. The neighbouring DNA regions of the gene encoding flavoredoxin were sequenced and characterised. Transcript analysis of the flavoredoxin gene resulted in a positive band corresponding to the size of the coding region, suggesting that flavoredoxin is encoded by a monocystronic...
Deletion of flavoredoxin gene in Desulfovibrio gigas reveals its participation ...
Broco, Manuela; Rousset, Marc; Oliveira, Solange; Rodrigues-Pousada, ClaudinaAbstract: The gene encoding Desulfovibrio gigas flavoredoxin was deleted to elucidate its physiological role in the sulfate metabolism. Disruption of flr gene strongly inhibited the reduction of thiosulfate and exhibited a reduced growth in the presence of sulfite with lactate as electron donor. The growth with sulfate was not however affected by the lack of this protein. Additionally, flr mutant cells revealed...
Expression of YAP4 in Saccharomyces cerevisiae under osmotic stress
Nevitt, Tracy; Pereira, Jorge; Azevedo, Dulce; Guerreiro, Paulo; Rodrigues-Pousada, ClaudinaYAP4, a member of the yeast activator protein (YAP) gene family, is induced in response to osmotic shock in the yeast Saccharomyces cerevisiae. The null mutant displays mild and moderate growth sensitivity at 0.4 M and 0.8 M NaCl respectively, a fact that led us to analyse YAP4 mRNA levels in the hog1 (high osmolarity glycerol) mutant. The data obtained show a complete abolition of YAP4 gene expression in this ...
Docking and electron transfer studies between rubredoxin and rubredoxin : oxyge...
Victor, Bruno; Vicente, João; Rodrigues, Rute; Oliveira, Solange; Rodrigues-Pousada, Claudina; Frazão, Carlos; Gomes, Cláudio; Teixeira, MiguelAbstract: The interaction and electron transfer (ET) between rubredoxin (Rd) and rubredoxin: oxygen oxidoreductase (ROO) from Desulfovibrio gigas is studied by molecular modelling techniques. Experimental kinetic assays using recombinant proteins show that the Rd reoxidation by ROO displays a bell-shaped dependence on ionic strength, suggesting a non-trivial electrostatic dependence of the interaction between t...
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