Encontrados 27 documentos, a visualizar página 1 de 3
mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditape...
Leite, Ricardo B.; Milan, Massimo; Coppe, Alessandro; Bortoluzzi, Stefania; dos Anjos, António; Reinhardt, Richard; Saavedra, Carlos; Patarnello, T.Background: The Grooved Carpet shell clam Ruditapes decussatus is the autochthonous European clam and the most appreciated from a gastronomic and economic point of view. The production is in decline due to several factors such as Perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam Ruditapes philippinarum. After we sequenced R. decussatus transcriptome we have desi...
Distinct patterns of notochord mineralization in zebrafish coincide with the lo...
Bensimon-Brito, A.; Cardeira, J.; Cancela, Leonor; Huysseune, Ann; Witten, PaulIn chondrichthyans, basal osteichthyans and tetrapods, vertebral bodies have cartilaginous anlagen that subsequently mineralize (chondrichthyans) or ossify (osteichthyans). Chondrocytes that form the vertebral centra derive from somites. In teleost fish, vertebral centrum formation starts in the absence of cartilage, through direct mineralization of the notochord sheath. In a second step, the notochord is surro...
Temporal and spatial expression patterns of pregnane X receptor and vitamin K e...
Fernández, Ignacio; Cancela, Leonor; Gavaia, Paulo J.Vitamin K (VK) is a liposoluble vitamin known to be essential for bone metabolism by two different pathways: (i) by its role as a coenzyme in the gamma-carboxylation of some skeletal proteins (e.g. osteocalcin (OC) and matrix Gla protein (MGP); Price et al., 1998); and (ii) through its role in skeletal gene transcription via binding to the pregnane X receptor (PXR; Azuma et al., 2010).
Matrix Gla protein expression: a complex process involving the use of alternati...
Cancela, Leonor; Laizé, Vincent; Conceição, N.; Tiago, Daniel M.; Maia, A. T.; Bensimon-Brito, A.; Gavaia, Paulo J.Matrix Gla protein (MGP) is a secreted vitamin K-dependent protein (VKD) located in the extracellular matrix and capable of binding calcium through its -carboxyglutamate residues. Although identified in 1983, transcriptional and post-transcriptional mechanisms regulating its expression remain unclear.
Skeletal quality in Senegalese sole: characterization of fish resulting from en...
Gavaia, Paulo J.; Dionísio, G.; Cardeira, J.; Cunha, M. E.; Pousão-Ferreira, P.; Conceição, L. E. C.; Cancela, LeonorThe Senegalese sole (Solea senegalensis) is characteristic from southern Europe and Mediterranean. It has recently been adapted for aquaculture production since it is well accepted by consumers and reaches high commercial values. After the initial description of the ontogenic events of skeletogenesis, systematic evaluation of the incidence of skeletal malformations by several groups revealed high levels of skel...
Global analysis of gene expression in mineralizing fish vertebra-derived cell l...
Tiago, Daniel M.; Laizé, Vincent; Bargelloni, Luca; Ferraresso, Serena; Romualdi, Chiara; Cancela, LeonorBackground Fish has been deemed suitable to study the complex mechanisms of vertebrate skeletogenesis and gilthead seabream (Sparus aurata), a marine teleost with acellular bone, has been successfully used in recent years to study the function and regulation of bone and cartilage related genes during development and in adult animals. Tools recently developed for gilthead seabream, e.g. mineralogenic cell lines ...
Transcriptome sequencing and microarray development for the Manila clam, Rudita...
Milan, Massimo; Coppe, Alessandro; Reinhardt, Richard; Cancela, Leonor; Leite, Ricardo B.; Saavedra, Carlos; Ciofi, Claudio; Chelazzi, GuidoAbstract Background The Manila clam, Ruditapes philippinarum, is one of the major aquaculture species in the world and a potential sentinel organism for monitoring the status of marine ecosystems. However, genomic resources for R. philippinarum are still extremely limited. Global analysis of gene expression profiles is increasingly used to evaluate the biological effects of various environmental stressors on aq...
Global analysis of gene expression in mineralizing fish vertebra-derived cell l...
Tiago, Daniel M.; Laizé, Vincent; Bargelloni, Luca; Ferraresso, Serena; Romualdi, Chiara; Cancela, LeonorAbstract Background Fish has been deemed suitable to study the complex mechanisms of vertebrate skeletogenesis and gilthead seabream (Sparus aurata), a marine teleost with acellular bone, has been successfully used in recent years to study the function and regulation of bone and cartilage related genes during development and in adult animals. Tools recently developed for gilthead seabream, e.g. mineralogenic ce...
Desenvolvimento de sistemas celulares de peixe adequados ao estudo da mineraliz...
Marques, C. L.; Rafael, M. S.; Tiago, Daniel M.; Cancela, Leonor; Laizé, VincentOs peixes foram recentemente reconhecidos como organismos modelo apropriados para o estudo da biologia de vertebrados, particularmente de processos relacionados com a mineralização tecidular e o desenvolvimento do esqueleto. Apesar de existirem alguns estudos in vivo, a identificação de mecanismos associados à calcificação em peixes tem sido prejudicada pelo facto de não existirem sistemas celulares para estudo...
Skeletal evaluation and technical improvements to decrease the incidence of ske...
Gavaia, Paulo J.; Richard, N.; Dâmaso, L.; Dinis, Maria Teresa; Pousão-Ferreira, P.; Engrola, S.; Conceição, L. E. C.; Cancela, LeonorThe appearance of skeletal deformities is a major problem associated to intensive, large-scale culture of fish species, affecting growth, development and ultimately, survival of produced fish. Although the mechanisms working behind it are still unclear, it is known that environmental, genetic and nutritional factors play fundamental roles. Given its importance as a vertebrate model to study skeleton-related dis...
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