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Spreading of HIV-1 subtype G and envB/gagG recombinant strains among injecting ...
Esteves, A; Parreira, R; Piedade, J; Venenno, T; Franco, M; Sousa, JG; Patrício, L; Brum, P; Costa, A; Canas-Ferreira, WWe have evaluated the genetic diversity of HIV-1 strains infecting injecting drug users (IDUs) in Lisbon, Portugal. Heteroduplex mobility assay and/or phylogenetic analysis revealed that env (C2V3C3 or gp41) subtype B is present in 63.7% of the 135 viral samples studied, followed by subtypes G (23.7%), A (6.7%), F (5.2%), and D (0.7%). Similar analysis of gag (p24/p7) performed on 91 of the specimens demonstrat...
Molecular epidemiology of HIV type 1 infection in Portugal: high prevalence of ...
Esteves, A; Parreira, R; Venenno, T; Franco, M; Piedade, J; Sousa, JG; Canas-Ferreira, WIn this study, we have investigated the diversity of current HIV-1 strains circulating in the metropolitan area of Lisbon, Portugal. A total of 217 HIV-1-positive blood samples, collected between October 1998 and December 2000, was genetically characterized in the gp120 C2V3C3 region (n = 205) or part of the gp41 N-terminal segment (n = 12) by heteroduplex mobility assay (HMA) and/or DNA sequencing. The HMA sub...
Active replication of hepatitis B virus (HBV) in HIV type 1 and in HIV type 2 i...
Barros,M.F.; Piedade,J.; Nunes,G.; Canas-Ferreira,W.; Silva,A.P.; Champalimaud,J.L.; Machado-Caetano,J.A.To evaluate the effect of concurrent infection by HIV on HBV infection or immunity, we have studied a group of 66 HIV1+ symptomatic Caucasian patients and another of 38 African HIV2+ asymptomatic individuals, concerning their HBV status: serological markers of infection and presence of HBV-DNA in serum, the last taken as sign of hepatitis B virus active replication, were monitored. HIV+ groups were compared wit...
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