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Development of stable flocculent Saccharomyces cerevisiae strain for continuous...
Oliveira, Carla Cristina Marques de; Teixeira, J. A.; Lima, Nelson; Silva, Nancy A. da; Domingues, LucíliaA flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger β-galactosidase in a continuous high-cell-density bioreactor. The δ-sequences from the yeast retrotransposon Ty1 were used as target sites for the integration of the β-galactosidase expression cassette. High-copy-number transformants were successfully obtained using the δ-integration system together with the dominant...
Data: 2007
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Origem: RepositóriUM - Universidade do Minho
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