Encontrados 5 documentos, a visualizar página 1 de 1
Performance of an indigenous β-mercaptoethanol-modified antigen in comparison w...
Semião-Santos, Saul; Veloso, Laura; Andrade, Paulo; Melo, Márcia; Martins, Luís; Marinho, Artur; Almeida, José; Campino, Lénea; Harith, AbdallahWe compared the performance of a locally produced b-mercaptoethanol-modified promastigote antigen (b-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different ...
Resposta imune na infecção por Leishmania infantum em modelo murino
Cabrita, Élia; Campino, Lenea; Maia, CarlaAs leishmanioses são doenças causadas por protozoários do género Leishmania que são parasitas intracelulares obrigatórios das células fagocíticas. O objectivo deste estudo foi caracterizar a infecção por Leishmania infantum em murganhos BALB/c inoculados por via intradérmica, analisando a evolução do parasitismo e as respostas imunitárias desenvolvidas. A carga parasitária foi determinada por PCR em tempo real....
Epidemiology of leishmaniases in Portugal.
Campino, Lenea; Maia, CarlaLeishmaniases, caused by the intracellular protozoan Leishmania infantum, are an endemic zoonosis in the Mediterranean basin. Dogs are considered the major host for these parasites, and the main reservoir for human visceral infection. Parasites are transmitted by the bite of phlebotomine sand flies, being Phlebotomus perniciosus and P. ariasi the proven vectors in Portugal. The global climate changes associated...
Studies in a co-infection murine model of Plasmodium chabaudi chabaudi and Leis...
Marques,Cláudia S; Rolão,Nuno; Centeno-Lima,Sónia; Lousada,Hélder; Maia,Carla; Campino,Lenea; Rosário,Virgílio E do; Silveira,HenriqueThis work aimed to study the T helper type 1/2 (Th1/Th2) cytokine profile in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum. Expression of interferon-gamma (IFN<FONT FACE=Symbol>-g</FONT>) and interleukin-4 (IL-4) was analyzed, in spleen and liver of C57BL/6 mice, by reverse transcriptase-polymerase chain reaction. High levels of IFN<FONT FACE=Symbol>-g</FONT> expression did...
Canine experimental infection: intradermal inoculation of Leishmania infantum p...
Santos-Gomes,Gabriela M; Campino,Lenea; Abranches,PedroFive mixed breed dogs were inoculated intradermally (ID) with cultured virulent stationary phase promastigotes of Leishmania infantum Nicole, 1908 stocks recently isolated. Parasite transformations in the skin of ID infected dogs were monitored from the moment of inoculation and for 48 h, by skin biopsies. Anti-Leishmania antibody levels were measured by indirect immunofluorescence assay, counterimmunoelectroph...
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