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Optimization of the culture medium composition using response surface methodolo...
Sampaio, Pedro N.; Calado, C.R.C.; Sousa, Lisete; Bressler, David C.; Pais, Maria Salomé; Fonseca, Luís PinaThe optimization of culture medium compositionwas carried out for improvement the recombinantcyprosin B production, an enzyme with high milk-clottingactivity. Response surface methodology (RSM) wasapplied to evaluate the effect of variables namely glucose,yeast extract (YE) and bactopeptone present in the culturemedium, used for recombinant cyprosin B production bytransformed Saccharomyces cerevisiae BJ1991 str...
Prediction of retention time of cutinases tagged with hydrophobic peptides in h...
Lienqueo, M.E.; Salazar, O.; Henriquez, K.; Calado, C.R.C.; Fonseca, L.P.; Cabral, J.M.SHydrophobic interaction chromatography (HIC) is an important technique for protein purification, which exploits the separation of proteins based on hydrophobic interactions between the stationary phase ligands and hydrophobic regions on the protein surface. One way of enhancing the purification efficiency by HIC is the addition of short sequences of peptide tags to the target protein by genetic engineering, whi...
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