Primordial Germ Cells (PGCs) identification and manipulation present considerable potential for hatchery practice and surrogate broodstocks. To carry out the PGCs characterization a specific molecular marker is required. The vasa gene is a good candidate to identify PGCs and others germinal cells (Nagasawa et al., 2009). The aim of this study was the cloning of the Solea senegalensis vasa cDNA and its expressio...
Tese de doutoramento, Ciências Jurídico-Económicas (Economia Política), Universidade de Lisboa, Faculdade de Direito, 2010. ; Inicialmente um tema científico, a questão climática alarga de forma gradual as suas fronteiras para assumir uma natureza política e jurídica, obrigando a uma abordagem multidisciplinar para compreender a sua autenticidade, fontes e impactos e para encontrar uma solução adequada a um pr...
One of the major obstacles to propagating dusky grouper, Epinephelus marginatus, is the difficulty of rearing the early larvae. We have successfully raised dusky grouper larvae in mesocosms using a mixed diet of endogenous plankton grown in the rearing tank and an exogenous supply of Brachionus plicatilis and Artemia sp. Newly hatched larvae at an initial density of 1.3 ind.l-1 were stocked in partially shaded ...
Immature fish and young females were sex reversed using hormonal induction in two consecutive years. In the first year one permanent male was obtained. Collected sperm of both years ranges from 30 μl to 300 μl and cell concentration varies from 3 to 6.5 x109 spermatozoa/ml. Atlantic dusky grouper can successfully be induced to functional males with 17α-methyltestosterone implants.
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