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Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood ne...
CECILE, FRANÇOIS; CASTAGNONE, CHANTAL; BOONHAM, NEIL; TOMLINSON, JENNY; LAWSON, REBECCA; HOCKLAND, SUE; QUILL, JAMES; VIEIRA, Paulo; MOTA, ManuelThe pinewood nematode (PWN), Bursaphelenchus xylophilus , is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe. A real-time PCR approach based on TaqMan chemistry was developed to detect this organism. Specific probe and primers were designed based on the sequence of the Msp I...
Data: 2007
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Origem: Repositório Científico da Universidade de Évora
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