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Detailed morphobiometric studies of Bursaphelenchus xylophilus and characterisa...
Penas, Ana Catarina; Bravo, Maria Antónia; Valadas, Vera; Mota, ManuelDetailed studies on Bursaphelenchus xylophilus are provided in this contribution. Comparative observations between field and cultured populations of this species demonstrated significant size differences: cultured specimens overall displayed larger size in all morphometric parameters. A principal component analysis (PCA) of the individuals undergoing moulting allowed their separation in four groups namely J2-J3...
Species of Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) and oth...
Penas, Ana Catarina; Bravo, Maria Antónia; Naves, Pedro; Bonifácio, Luís; Sousa, Edmundo; Mota, ManuelInsects associated with maritime pine, Pinus pinaster, in Portugal were collected and screened for the presence of Bursaphelenchus species. Nematodes were identified using Internal Transcribed Spacers-Restriction Fragment Length Polymorphism (ITS-RFLP) analysis of dauer juveniles and morphological identification of adults that developed from dauer juveniles on fungal cultures or on cultures in pine wood segment...
Species of Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) associa...
Penas, Ana Catarina; Correia, Petra; Bravo, Maria Antónia; Mota, Manuel; Tenreiro, RogérioSummary – Species of Bursaphelenchus associated with maritime pine, Pinus pinaster, from Portugal – within and outside the quarantine restricted demarcated zone of B. xylophilus – are described and characterised both morphologically (LM and SEM) and with the use of molecular biology (ITS-RFLP).A new staining method for spicules is proposed. Species include B. hellenicus, B. hylobianum, B. leoni, B. pinophilus, ...
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